LY6C + LY6G Antikörper

Produktnummer ABIN967573

Produktdetails

Target: LY6C + LY6G

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Cytotoxicity Test (CyTox)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not Reported

Klon: RB6-8C5

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: For immunohistochemistry, we recommend using purified RB6-8C5 antibody in our special formulation for IHC.

Kommentare:

Related Products: ABIN967573

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen

Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).

Rakhmilevich: "Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes." in: Journal of leukocyte biology, Vol. 57, Issue 6, pp. 827-31, (1995) (PubMed).

Conlan, North: "Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 259-68, (1994) (PubMed).

Czuprynski, Brown, Maroushek, Wagner, Steinberg: "Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 4, pp. 1836-46, (1994) (PubMed).

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, (1993) (PubMed).

Tepper, Coffman, Leder: "An eosinophil-dependent mechanism for the antitumor effect of interleukin-4." in: Science (New York, N.Y.), Vol. 257, Issue 5069, pp. 548-51, (1992) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).

Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, (1989) (PubMed).

Antigendetails

Target: LY6C + LY6G

Andere Bezeichnung: Ly-6G and Ly-6C

Hintergrund: The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the lineage cocktail used in studies of hematopoietic lineages. The mAb 1A8 specifically recognizes Ly-6G, but not Ly-6C. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Gr-1