SREBF2 Antikörper (AA 833-1141)

Produktnummer ABIN967564

Produktdetails anti-SREBF2 Antikörper

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Bindungsspezifität: AA 833-1141

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SREBF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SREBP-2 aa. 833-1141

Klon: IgG-1C6

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The IgG-1C6 antibody may be used for western blot analysis (2 µg/ml) and immunoprecipitation (2 µg antibody/300-500 µg of total protein lysate). U-937 human histiocytic lymphoma cells (ATCC CRL-1593) or HeLa human cervical carcinoma cells (ATCC CCL-2) are recommended as additional positive controls.

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-SREBF2 Antikörper (ABIN967564)

Sakai, Nohturfft, Cheng, Ho, Brown, Goldstein: "Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 20213-21, (1997) (PubMed).

Hua, Sakai, Ho, Goldstein, Brown: "Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29422-7, (1996) (PubMed).

Wang, Zelenski, Yang, Sakai, Brown, Goldstein: "Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis." in: The EMBO journal, Vol. 15, Issue 5, pp. 1012-20, (1996) (PubMed).

Wang, Pai, Wiedenfeld, Medina, Slaughter, Goldstein, Brown: "Purification of an interleukin-1 beta converting enzyme-related cysteine protease that cleaves sterol regulatory element-binding proteins between the leucine zipper and transmembrane domains." in: The Journal of biological chemistry, Vol. 270, Issue 30, pp. 18044-50, (1995) (PubMed).

Details zu SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Andere Bezeichnung: SREBP-2 (SREBF2 Produkte)

Synonyme: SREBF2 antikoerper, srebp2 antikoerper, SREBP2 antikoerper, zgc:158371 antikoerper, AI608257 antikoerper, SREBP-2 antikoerper, SREBP2gc antikoerper, bHLHd2 antikoerper, lop13 antikoerper, nuc antikoerper, Srebf2_retired antikoerper, sterol regulatory element binding transcription factor 2 antikoerper, sterol regulatory element binding factor 2 antikoerper, sterol regulatory element binding transcription factor 2 L homeolog antikoerper, SREBF2 antikoerper, srebf2 antikoerper, Srebf2 antikoerper, srebf2.L antikoerper

Hintergrund: SREBP-1 and -2 (sterol-regulatory element binding proteins-1 and -2) are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, a ~480-500 amino acid, N-terminal cleavage fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis. Proteases which cleave SREBPs have been identified and include SCA (SREBP-cleavage activity), as well as caspase-3, a key regulator of apoptotic pathways. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes. However, sterol-regulated vs. apoptosis-associated cleavage of SREBP proteins appears to be independently regulated. On SDS-PAGE, sterol-regulated cleavage fragments of SREBP proteins migrate more slowly (i.e., higher molecular weight) than do staurosporin-induced fragments. In addition, staurosporin-induced SREBP cleavage products may appear as a doublet, with the upper band representing a phosphorylated form of SREBP. On SDS-PAGE, full length, precursor forms of SREBP-1 and -2 migrate at ~125 kD, while proteolytic cleavage fragments may be observed as a cluster of bands between 60 - 70 kDa. The IgG-1C6 antibody recognizes human SREBP-2. The antibody recognizes the C-terminal of human SREBP-2. A fusion protein containing C-terminal amino acids 833-1141 of human SREBP-2, was used as immunogen. The antibody recognizes both the 125 kDa precursor and the 60-70 kDa COOH-terminal cleaved forms of SREBP-2.
Synonyms: Sterol Regulatory Element-Binding Factor 1, SREBF1

Pathways: Regulation of Lipid Metabolism by PPARalpha