Cytochrome C Antikörper

Produktnummer ABIN967529

Produktdetails anti-Cytochrome C Antikörper

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human, Ratte, Maus, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Kreuzreaktivität: Human, Maus, Ratte (Rattus), Pferd

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Synthetic Peptides of Pigeon Cytochrome C

Klon: 7H8-2C12

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Applications include western blot analysis (1-2 µg/ml). Clone 7H8.2C12 is not useful for immunoprecipitation. However, clone 6H2.B4 is useful for immunoprecipitation of Cytochrome c.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-Cytochrome C Antikörper (ABIN967529)

Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, (1996) (PubMed).

Goshorn, Retzel, Jemmerson: "Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c." in: The Journal of biological chemistry, Vol. 266, Issue 4, pp. 2134-42, (1991) (PubMed).

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: Cytochrome c (CYCS Produkte)

Synonyme: CYC antikoerper, HCS antikoerper, THC4 antikoerper, cyct antikoerper, cyc antikoerper, hcs antikoerper, CYCS antikoerper, cytc antikoerper, CYCSA antikoerper, CG17903 antikoerper, Cyt C antikoerper, Cyt c antikoerper, Cyt-c2 antikoerper, CytC-2 antikoerper, Cytc antikoerper, Cytc-p antikoerper, DC4 antikoerper, DMc01 antikoerper, Dmel\\CG17903 antikoerper, cyt c antikoerper, cyt c-p antikoerper, cyt-c-p antikoerper, cyt-c2 antikoerper, cyt.c antikoerper, cytochrome c antikoerper, dc4 antikoerper, pDMc01 antikoerper, wu:fk52b01 antikoerper, zgc:86706 antikoerper, ENSMUSG00000058927 antikoerper, cytochrome c, somatic antikoerper, cytochrome c, somatic S homeolog antikoerper, Cytochrome c proximal antikoerper, cytochrome c, somatic b antikoerper, cytochrome c, somatic pseudogene antikoerper, cytochrome c-like antikoerper, cytochrome c antikoerper, CYCS antikoerper, cycs.S antikoerper, cycs antikoerper, Cycs antikoerper, Cyt-c-p antikoerper, cycsb antikoerper, Gm10053 antikoerper, LOC100724240 antikoerper, CYC antikoerper, LOC100847700 antikoerper, LOC101107954 antikoerper, LOC106829744 antikoerper, LOC106992390 antikoerper, LOC102085712 antikoerper

Hintergrund: A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four groups (a, b, c, and d) according to spectrochemical characteristics, and there are five cytochromes between Coenzyme QH2 and O2 in the electron transport chain. Cytochrome c is a water-soluble peripheral membrane protein of the mitochondria and transports electrons from the Coenzyme QH2 - Cytochrome c reductase complex to the Cytochrome c oxidase complex. Recently it has been suggested that Cytochrome c may be required for cells to undergo apoptosis. Mitochondria may be involved in apoptosis by releasing Cytochrome c to the cytosol where it functions in the activation of Caspase 3 (CPP32), an ICE family molecule that has been shown to trigger apoptosis when activated. In the cytoplasm Cytochrome C might exist in polymeric forms, appearing as a 58-60 kDa band by western blot analysis, rather than the monomeric form. Monomeric Cytochrome c migrates at a reduced molecular weight of 15 kDa.
Clone 7H8.2C12 recognizes the denatured form of pigeon, horse, rat, mouse and human Cytochrome c. It does not recognize the native form of Cytochrome c. Clone 7H8.2C12 recognizes an epitope within amino acids 93-104 (inclusive) of pigeon Cytochrome C, based on competitive ELISA results. Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon Cytochrome c were used as immunogens. Clone 7H8.2C12 is used for western blot detection of Cytochrome c. Clone 6H2.B4, is used for immunoprecipitation. Cell lines P388D1 mouse lymphoma (ATCC CCL-46), HeLa human carcinoma (ATCC CCL-2), Jurkat T leukemia (ATCC TIB-152), and NIH/3T3 mouse fibroblast cells (ATCC CRL-1658) may be used as positive control cell lysates.
This antibody is routinely tested by western blot analysis.

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity