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PIK3R1 Antikörper

PI3K p85a Reaktivität: Human, Rind (Kuh), Affe WB, ELISA, IP Wirt: Maus Monoclonal U15 unconjugated
Produktnummer ABIN967523
  • Target Alle PIK3R1 (PI3K p85a) Antikörper anzeigen
    PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
    Reaktivität
    • 140
    • 97
    • 72
    • 18
    • 17
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Rind (Kuh), Affe
    Wirt
    • 124
    • 32
    Maus
    Klonalität
    • 108
    • 48
    Monoklonal
    Konjugat
    • 80
    • 11
    • 11
    • 7
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser PIK3R1 Antikörper ist unkonjugiert
    Applikation
    • 122
    • 59
    • 41
    • 28
    • 25
    • 18
    • 17
    • 16
    • 14
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Marke
    BD Pharmingen™
    Kreuzreaktivität
    Human, Affe
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Recombinant cow p85alpha
    Klon
    U15
    Isotyp
    IgG1
    Top Product
    Discover our top product PI3K p85a Primärantikörper
  • Applikationshinweise
    Clone U15 was originally characterized by ELISA, immunoprecipitation, and western blot analysis. Applications include immunoprecipitation (1-2 µg/1x10^6 cells) and western blot analysis (1-2 µg/ml). In immunoprecipitation, U15 can bring down an active kinase. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Shoelson, Sivaraja, Williams, Hu, Schlessinger, Weiss: "Specific phosphopeptide binding regulates a conformational change in the PI 3-kinase SH2 domain associated with enzyme activation." in: The EMBO journal, Vol. 12, Issue 2, pp. 795-802, (1993) (PubMed).

    Whitman, Downes, Keeler, Keller, Cantley: "Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate." in: Nature, Vol. 332, Issue 6165, pp. 644-6, (1988) (PubMed).

  • Target
    PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
    Andere Bezeichnung
    pi 3-Kinase p85 alpha (PI3K p85a Produkte)
    Synonyme
    PI3CG antikoerper, PI3K antikoerper, PI3Kgamma antikoerper, PIK3 antikoerper, AGM7 antikoerper, GRB1 antikoerper, p85 antikoerper, p85-ALPHA antikoerper, PI3KA antikoerper, AA414921 antikoerper, C530050K14 antikoerper, p50alpha antikoerper, p55alpha antikoerper, p85alpha antikoerper, wu:fb65b05 antikoerper, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma antikoerper, phosphoinositide-3-kinase regulatory subunit 1 antikoerper, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta antikoerper, phosphoinositide-3-kinase, regulatory subunit 1 (alpha) antikoerper, PIK3CG antikoerper, PIK3R1 antikoerper, Pik3r1 antikoerper, Pik3cb antikoerper, pik3r1 antikoerper
    Hintergrund
    Phosphatidylinositol 3-kinase (PI3-kinase) is a universal enzyme associated with receptor signaling pathways. It generates second messenger phospholipids by phosphorylating the D-3 position of inositol phospholipids including phosphatidylinositol (PI), PI-4-phosphate, and PI-4, 5-biphosphate. The enzyme exists as a heterodimer composed of regulatory 85 kDa (p85) and catalytic 110 kDa (p110) subunits. The p85 subunit contains two SH2 (src-homology 2) domains and an SH3 domain. The catalytic activity of the p110 subunit is stimulated when the p85 regulatory subunit binds, through its SH2 domains, to activated receptor and non-receptor tyrosine kinases. Two p85 isoforms have been described, p85a and p85ß. Both isoforms bind to activated receptors and each may be responsible for mediating a subset of PI3-kinase interactions. Clone U15 recognizes the p85 regulatory subunit of PI3 kinase (p85alpha). It reacts with human, monkey, and cow PI3 kinase. It does not cross-react with mouse or rat PI3 kinase. Recombinant cow p85alpha was used as immunogen. The epitope has been mapped to the inter-SH2 spacer region of p85alpha. The antibody will block lipids binding to this region.
    Molekulargewicht
    85 kDa
    Pathways
    T-Zell Rezeptor Signalweg, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effekt
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