CD247 Antikörper (AA 153-164)

Produktnummer ABIN967517

Produktdetails anti-CD247 Antikörper

Target: CD247 (CD247 Molecule (CD247))

Bindungsspezifität: AA 153-164

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD247 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD3zeta synthetic peptide aa. 153-164

Klon: 1D4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Applications include immunoprecipitation and western blot analysis (1-2 µg/ml). Jurkat T cells (ATCC TIB 152) are suggested as a positive control.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD247 Antikörper (ABIN967517)

Hall, Sancho, Terhorst: "Reconstitution of T cell receptor zeta-mediated calcium mobilization in nonlymphoid cells." in: Science (New York, N.Y.), Vol. 261, Issue 5123, pp. 915-8, (1993) (PubMed).

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, (1993) (PubMed).

Sancho, Franco, Chatila, Hall, Terhorst: "The T cell receptor associated CD3-epsilon protein is phosphorylated upon T cell activation in the two tyrosine residues of a conserved signal transduction motif." in: European journal of immunology, Vol. 23, Issue 7, pp. 1636-42, (1993) (PubMed).

Details zu CD247

Target: CD247 (CD247 Molecule (CD247))

Andere Bezeichnung: CD247 (CD247 Produkte)

Synonyme: CD3-ZETA antikoerper, CD3H antikoerper, CD3Q antikoerper, CD3Z antikoerper, T3Z antikoerper, TCRZ antikoerper, 4930549J05Rik antikoerper, A430104F18Rik antikoerper, AW552088 antikoerper, Cd3 antikoerper, Cd3-eta antikoerper, Cd3-zeta antikoerper, Cd3h antikoerper, Cd3z antikoerper, Cd3zeta antikoerper, T3z antikoerper, Tcrk antikoerper, Tcrz antikoerper, LOC100136514 antikoerper, LOC100136549 antikoerper, LOC100305023 antikoerper, CD247 antikoerper, TCRzeta antikoerper, CD247 molecule antikoerper, CD247 antigen antikoerper, CD3zeta-2 antikoerper, CD3zeta-1 antikoerper, Cd247 molecule antikoerper, CD247 antikoerper, Cd247 antikoerper, cd3z antikoerper, cd247 antikoerper

Hintergrund: The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains, gamma (gamma), delta (delta), epsilon (epsilon), zeta (zeta), and p21. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer), and 21 kD respectively. 1D4 recognizes human but not mouse CD3zeta. It is most efficient at recognizing the phosphorylated form of CD3zeta (21 kD) but will also bind the 16 kD unphosphorylated monomer as well as higher molecular weight dimers. A synthetic peptide corresponding to amino acids 153 to 164 of human CD3zeta was used as immunogen.
Synonyms: CD3zeta chain

Molekulargewicht: 21 kDa

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway