Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MSH2 Antikörper

MSH2 Reaktivität: Human WB, IHC (p), IHC (fro) Wirt: Maus Monoclonal G219-1129 unconjugated
Produktnummer ABIN967509
  • Target Alle MSH2 Antikörper anzeigen
    MSH2 (Mismatch Repair Protein 2 (MSH2))
    Reaktivität
    • 80
    • 41
    • 19
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 76
    • 18
    • 1
    Maus
    Klonalität
    • 71
    • 25
    Monoklonal
    Konjugat
    • 61
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MSH2 Antikörper ist unkonjugiert
    Applikation
    • 60
    • 37
    • 27
    • 22
    • 21
    • 14
    • 13
    • 12
    • 12
    • 8
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Marke
    BD Pharmingen™
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Recombinant Human MSH2 Protein
    Klon
    G219-1129
    Isotyp
    IgG1
    Top Product
    Discover our top product MSH2 Primärantikörper
  • Applikationshinweise
    For IHC, intestine is suggested as a positive control. Staining is typically seen in the crypts of Lieberkuhn, similar to that described by others. Staining is primarily nuclear, but may also be observed in the cytoplasm.
    Kommentare

    Related Products: ABIN968533

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Wilson, Ewel, Duguid, Eble, Lescoe, Fishel, Kelley: "Differential cellular expression of the human MSH2 repair enzyme in small and large intestine." in: Cancer research, Vol. 55, Issue 22, pp. 5146-50, (1995) (PubMed).

    Cleaver: "It was a very good year for DNA repair." in: Cell, Vol. 76, Issue 1, pp. 1-4, (1994) (PubMed).

    Fishel, Lescoe, Rao, Copeland, Jenkins, Garber, Kane, Kolodner: "The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer." in: Cell, Vol. 75, Issue 5, pp. 1027-38, (1994) (PubMed).

    Leach, Nicolaides, Papadopoulos, Liu, Jen, Parsons, Peltomäki, Sistonen, Aaltonen, Nyström-Lahti: "Mutations of a mutS homolog in hereditary nonpolyposis colorectal cancer." in: Cell, Vol. 75, Issue 6, pp. 1215-25, (1994) (PubMed).

    Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, (1994) (PubMed).

    Prolla, Pang, Alani, Kolodner, Liskay: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." in: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, (1994) (PubMed).

    Kramer, Kramer, Williamson, Fogel: "Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB." in: Journal of bacteriology, Vol. 171, Issue 10, pp. 5339-46, (1989) (PubMed).

    Su, Modrich: "Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5057-61, (1986) (PubMed).

  • Target
    MSH2 (Mismatch Repair Protein 2 (MSH2))
    Andere Bezeichnung
    MSH2 (MSH2 Produkte)
    Synonyme
    COCA1 antikoerper, FCC1 antikoerper, HNPCC antikoerper, HNPCC1 antikoerper, LCFS2 antikoerper, AI788990 antikoerper, wu:fc06b02 antikoerper, wu:fc13e09 antikoerper, zgc:55333 antikoerper, ATMSH2 antikoerper, MUTS homolog 2 antikoerper, msh2 antikoerper, mutS homolog 2 antikoerper, mutS homolog 2 (E. coli) antikoerper, MUTS homolog 2 antikoerper, mutS homolog 2 L homeolog antikoerper, MutS protein homolog 2 antikoerper, MSH2 antikoerper, Msh2 antikoerper, msh2 antikoerper, msh2.L antikoerper
    Hintergrund
    The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. The human homologs of DNA mismatch repair enzymes MLH1, PMS2, and MSH2 have recently been cloned. G219-1129 recognizes human MSH-2. A recombinant full-length human MSH2 protein was used as immunogen.
    Molekulargewicht
    102 kDa
    Pathways
    DNA Reparatur, Production of Molecular Mediator of Immune Response
Sie sind hier:
Kundenservice