GFAP Antikörper

Produktnummer ABIN967500

Produktdetails anti-GFAP Antikörper

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human, Ratte, Maus, Schwein, Rind (Kuh), Huhn, Kaninchen, Meerschweinchen, Schaf, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Kreuzreaktivität: Human, Maus, Rind (Kuh), Schaf, Hund, Schwein, Kaninchen, Meerschweinchen, Huhn, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow spinal cord homogenate

Klon: 1B4

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Additional GFAP specific antibodies: clone 2E1 (ABIN967501), 4A11 (ABIN967499), and clones 1B4, 4A11, 2E1 combined and available as a cocktail” (ABIN967502). Applications include indirect immunofluorescence of tissue-cultured cells, immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (25 µg/ml), and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control

Kommentare:

Related Products: ABIN967501, ABIN967499, ABIN967502

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-GFAP Antikörper (ABIN967500)

McLendon, Bigner: "Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).

McLendon, Burger, Pegram, Eng, Bigner: "The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues." in: Journal of neuropathology and experimental neurology, Vol. 45, Issue 6, pp. 692-703, (1986) (PubMed).

Pegram, Eng, Wikstrand, McComb, Lee, Bigner: "Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species." in: Neurochemical pathology, Vol. 3, Issue 2, pp. 119-38, (1985) (PubMed).

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP (GFAP Produkte)

Synonyme: GFAP antikoerper, AI836096 antikoerper, cb345 antikoerper, etID36982.3 antikoerper, gfapl antikoerper, wu:fb34h11 antikoerper, wu:fk42c12 antikoerper, xx:af506734 antikoerper, zgc:110485 antikoerper, glial fibrillary acidic protein antikoerper, GFAP antikoerper, LOC100136168 antikoerper, gfap antikoerper, Gfap antikoerper

Hintergrund: GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. We offer a panel ofnmonoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, cow, sheep, dog, pig, rabbit, guinea pig and chicken. 1B4 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells.
The antibody is routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure).

Molekulargewicht: 50 kDa