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p53 Antikörper

TP53 Reaktivität: Human, Affe, Rind (Kuh) WB, IP, IHC (f), IHC (fro), ICS Wirt: Maus Monoclonal DO unconjugated
Produktnummer ABIN967458
  • Target Alle p53 (TP53) Antikörper anzeigen
    p53 (TP53) (Tumor Protein P53 (TP53))
    Reaktivität
    • 900
    • 273
    • 223
    • 112
    • 47
    • 35
    • 33
    • 19
    • 10
    • 8
    • 8
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Affe, Rind (Kuh)
    Wirt
    • 678
    • 238
    • 6
    • 2
    • 1
    • 1
    Maus
    Klonalität
    • 612
    • 313
    • 1
    Monoklonal
    Konjugat
    • 518
    • 49
    • 40
    • 26
    • 25
    • 25
    • 24
    • 24
    • 23
    • 22
    • 22
    • 13
    • 13
    • 13
    • 13
    • 13
    • 13
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser p53 Antikörper ist unkonjugiert
    Applikation
    • 725
    • 336
    • 257
    • 217
    • 210
    • 151
    • 128
    • 124
    • 119
    • 65
    • 61
    • 34
    • 20
    • 17
    • 13
    • 12
    • 12
    • 10
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS)
    Marke
    BD Pharmingen™
    Kreuzreaktivität
    Affe, Rind (Kuh)
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Recombinant p53 protein
    Klon
    DO
    Isotyp
    IgG2b
    Top Product
    Discover our top product TP53 Primärantikörper
  • Applikationshinweise
    Positive control cell lines include SK-BR-3 breast carcinoma cells (ATCC HTB-30), A431 epidermal carcinoma cells (ATCC CRL-1555) and CEM human leukemia cells (ATCC CCL-119). MCF-7 breast carcinoma cells (ATCC HTB-22) are suggested as a negative control. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Baas, Mulder, Offerhaus, Vogelstein, Hamilton: "An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms." in: The Journal of pathology, Vol. 172, Issue 1, pp. 5-12, (1994) (PubMed).

    Cripps, Purdie, Carder, White, Komine, Bird, Wyllie: "A study of stabilisation of p53 protein versus point mutation in colorectal carcinoma." in: Oncogene, Vol. 9, Issue 9, pp. 2739-43, (1994) (PubMed).

    Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, (1994) (PubMed).

    Lambkin, Mothersill, Kelehan: "Variations in immunohistochemical detection of p53 protein overexpression in cervical carcinomas with different antibodies and methods of detection." in: The Journal of pathology, Vol. 172, Issue 1, pp. 13-8, (1994) (PubMed).

    Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, (1992) (PubMed).

    Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, (1991) (PubMed).

  • Target
    p53 (TP53) (Tumor Protein P53 (TP53))
    Andere Bezeichnung
    p53 (TP53 Produkte)
    Synonyme
    BCC7 antikoerper, LFS1 antikoerper, P53 antikoerper, TRP53 antikoerper, p53 antikoerper, Trp53 antikoerper, brp53 antikoerper, drp53 antikoerper, etID22686.5 antikoerper, fb40d06 antikoerper, wu:fb40d06 antikoerper, zgc:111919 antikoerper, Tp53 antikoerper, bbl antikoerper, bfy antikoerper, bhy antikoerper, p44 antikoerper, tp53 antikoerper, Xp53 antikoerper, TP53 antikoerper, tumor protein p53 antikoerper, cellular tumor antigen p53 antikoerper, transformation related protein 53 antikoerper, tumor protein p53 L homeolog antikoerper, TP53 antikoerper, CpipJ_CPIJ002758 antikoerper, Tp53 antikoerper, tp53 antikoerper, Trp53 antikoerper, tp53.L antikoerper
    Hintergrund
    P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. The DO-7 antibody recognizes human wildtype and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-7 recognizes an epitope between amino acids 1-45 of known forms of human p53. Human recombinant p53 protein was used as immunogen. Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies (mAbs) in normal tissues.
    Molekulargewicht
    53 kDa
    Pathways
    p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt
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