Clusterin Antikörper

Produktnummer ABIN967369

Produktdetails anti-Clusterin Antikörper

Target: Clusterin (CLU)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Clusterin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: E5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry:
The E5 antibody, specific for human clusterin, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Tissue tested was human tonsil. The antibody stains dendritic cells in the follicle and basement membrane of epithelial cells. The isotype control recommended for use with this antibody is purified mouse IgG1. For optimal indirect immunohistochemical staining, the E5 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB Detection System. More conveniently, the Anti-Mouse Ig HRP Detection Kit, which contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate, can be used for staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Clusterin Antikörper (ABIN967369)

Wellmann, Thieblemont, Pittaluga, Sakai, Jaffe, Siebert, Raffeld: "Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas." in: Blood, Vol. 96, Issue 2, pp. 398-404, (2000) (PubMed).

Rosenberg, Silkensen: "Clusterin: physiologic and pathophysiologic considerations." in: The international journal of biochemistry & cell biology, Vol. 27, Issue 7, pp. 633-45, (1995) (PubMed).

Murphy, Kirszbaum, Walker, dApice: "SP-40,40, a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis." in: The Journal of clinical investigation, Vol. 81, Issue 6, pp. 1858-64, (1988) (PubMed).

Details zu Clusterin

Target: Clusterin (CLU)

Andere Bezeichnung: Clusterin (CLU Produkte)

Synonyme: LOC100136120 antikoerper, clu antikoerper, MGC53055 antikoerper, zgc:73352 antikoerper, xclu antikoerper, MGC89163 antikoerper, CLU antikoerper, APO-J antikoerper, APOJ antikoerper, CLI antikoerper, CLU1 antikoerper, CLU2 antikoerper, KUB1 antikoerper, NA1/NA2 antikoerper, SGP-2 antikoerper, SGP2 antikoerper, SP-40 antikoerper, TRPM-2 antikoerper, TRPM2 antikoerper, DAG antikoerper, RATTRPM2B antikoerper, SP40 antikoerper, TRPM2B antikoerper, Trpm2 antikoerper, Trpmb antikoerper, AI893575 antikoerper, ApoJ antikoerper, Cli antikoerper, D14Ucla3 antikoerper, Sgp-2 antikoerper, Sgp2 antikoerper, Sugp-2 antikoerper, GP80 antikoerper, gpIII antikoerper, 40 antikoerper, clusterin-1 antikoerper, clusterin L homeolog antikoerper, clusterin antikoerper, Clusterin antikoerper, LOC100136120 antikoerper, clu.L antikoerper, clu antikoerper, CLU antikoerper, clus antikoerper, Clu antikoerper, LOC101113728 antikoerper

Hintergrund: The E5 antibody reacts with human clusterin. Clusterin was originally isolated and characterized from glomerular immune deposits of patients with renal glomerulonephritis. Clusterin, an 80 kD disulfide-linked, heterodimeric glycoprotein (previously named SP-40,40 [serum protein 40 kD,40kD]) was shown to be part of the SC5b-9 complex of complement, yet separate and distinct from the S protein. Clusterins have since been identified in a number of mammalian species and have been implicated in diverse cellular activities, however, the exact role remains to be elucidated.

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling