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His Tag Antikörper

Name: His Tag Antikörper
SKU: ABIN967362
Price: 196.9 EUR
Availability: InStock

WB, IP Wirt: Maus Monoclonal F24-796 unconjugated

1 image

(1 reference)

Produktnummer ABIN967362

Datenblatt (PDF)

Target Alle His Tag Antikörper anzeigen

His Tag

Reaktivität

Bitte anfragen
Wirt
94

56

12

9

8

7

2

1
Maus
Passende Sekundärantikörper
Klonalität
107

69

10
Monoklonal
Konjugat
91

23

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser His Tag Antikörper ist unkonjugiert
Applikation
153

103

38

28

25

17

14

14

14

13

13

7

7

6

6

5

4

4

3

3

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP)

Marke

BD Pharmingen™

Sequenz

HHHHHH

Produktmerkmale

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon

F24-796

Isotyp

IgG2b

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Applikationshinweise

An E. coli produced recombinant 6xHis control lysate is provided as a positive control. Store lysates at -20°C.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store undiluted at 4°C.
Hochuli, Döbeli, Schacher: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, (1988) (PubMed).
Target

His Tag

Andere Bezeichnung

6xHis (His Tag Produkte)

Substanzklasse

Tag

Hintergrund

The engineering of a short epitope tag to a recombinant protein provides a very convenient method for detecting and isolating the protein of interest. For example, the placement of a series of 6 consecutive histidine residues that are incorporated at either end of the recombinant fusion protein facilitates the purification of that protein. Purification can be easily accomplished by metal chelate affinity chromatography using a metal resin column. The 6xHis monoclonal antibody can be used to detect the epitope-tagged protein of interest.