Keratin 10/13 Antikörper

Produktnummer ABIN965954

Produktdetails

Target: Keratin 10/13

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Concentrated.

Immunogen: Cytoskeleton preparation from HeLa Cells

Klon: B28-1 (DE-K13)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with Auto ProbeIII. The optimal conditions should be determined by the individual laboratory. Enzymatic predigestion is not recommended.

Kommentare:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Squamous Granulocytes Carcinomas, Esophagus, or Tongue

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.4 mg/ml

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: Keratin 10/13

Andere Bezeichnung: Cytokeratin 10+13 (56.5 & 53kDa)

Hintergrund: This antibody reacts with Cytokeratin 10 and 13 (56.5 and 53 kD) polypeptide. On formalin-fixed,Paraffinembedded tissue sections, this antibody recognizes only one of the two cytokeratins: Cytokeratin 13, that is found in non-squamous stratified epithelia.