Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) Antikörper

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8)

Synonyme: CD30, Ki-1, D1S166E, Ki, Cd30, RP23-306F22.1, TNFRSF8, TNFSF8

Klonalität: Monoklonal (Ber-H2)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN96578

Produktbeschreibung

Weitere Bezeichnung: CD30

Immunogen: BALB/C mice were injected with L428 cell line cells.

Format: Supernatant (prediluted)

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: Ber-H2

Spezifität: This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.

Anwendungen

Applikationshinweise: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 10.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Konzentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: CD Antigene

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Pallesen: "The diagnostic significance of the CD30 (Ki-1) antigen." in: Histopathology, Vol. 16, Issue 4, pp. 409-13, 1990 (PubMed).

Schwarting, Gerdes, Duerkop et al.: "BER-H2: a new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope." in: Blood, Vol. 74, Issue 5, pp. 1678-89, 1989 (PubMed).

Pallesen, Hamilton-Dutoit: "Ki-1 (CD30) antigen is regularly expressed by tumor cells of embryonal carcinoma." in: The American journal of pathology, Vol. 133, Issue 3, pp. 446-50, 1989 (PubMed).