LYZ Antikörper (AA 1-129)

Produktnummer ABIN964705

Produktdetails anti-LYZ Antikörper

Target: LYZ (Lysozyme (LYZ))

Bindungsspezifität: AA 1-129

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYZ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This purified antibody is directed against lysozyme from hen egg white protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White). A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.

Produktmerkmale: Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of beta-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme ''c'' is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme ''g''. Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.

Sterilität: Sterile filtered

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Immunogen Type: NativeProtein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: LYZ

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/ml

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Details zu LYZ

Target: LYZ (Lysozyme (LYZ))

Andere Bezeichnung: Lysozyme (LYZ Produkte)

Synonyme: LZM antikoerper, 1 antikoerper, LYZC antikoerper, lys-C antikoerper, zgc:136734 antikoerper, BmLys antikoerper, LYS antikoerper, LZ antikoerper, LYZS antikoerper, lysozyme C antikoerper, LYZ antikoerper, lysozyme antikoerper, lysozyme antikoerper, lysozyme (renal amyloidosis) antikoerper, lysozyme L homeolog antikoerper, serum lysozyme antikoerper, LYZ antikoerper, lyz antikoerper, Lzm antikoerper, LOC781146 antikoerper, lyz.L antikoerper, S-LZ antikoerper

Hintergrund: Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of β-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers.  Lysozyme is common in animals and plants.  In birds, lysozyme is also an exceptionally abundant protein in egg whites.  Its biological function in fowl eggs is unclear.  Hen egg white lysozyme ''c'' is the most commonly studied form and source of the enzyme.  Lysozyme from domestic goose is designated lysozyme ''g''.  Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis.  Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.
Synonyms: 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, lysosyme antibody, Lysozyme C antibody, Lysozyme C precursor antibody, Lyz antibody, LZM antibody

Gen-ID: 395708, 47825389

UniProt: Q6LEL2