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V5 Epitope Tag Antikörper

WB, ELISA, IP, IF, FACS, ICC Wirt: Maus Monoclonal 4C12E11 unconjugated
Produktnummer ABIN962847
  • Target Alle V5 Epitope Tag Produkte
    V5 Epitope Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 60
    • 40
    • 10
    • 7
    • 6
    • 2
    • 1
    Maus
    Klonalität
    • 57
    • 56
    • 10
    Monoklonal
    Konjugat
    • 65
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser V5 Epitope Tag Antikörper ist unkonjugiert
    Applikation
    • 100
    • 58
    • 43
    • 43
    • 31
    • 30
    • 13
    • 9
    • 7
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Marke
    THE™
    Sequenz
    GKPIPNPLLG LDST
    Spezifität
    THETM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
    Produktmerkmale
    THETM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
    Aufreinigung
    Protein A affinity column
    Immunogen
    V5 tag peptide conjugated - KLH
    Klon
    4C12E11
    Isotyp
    IgG2a
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.1 µg/mL
    Western blot: 0.2-1 µg/mL
    Immunoprecipitation: 1-5 µg/mL
    FACS: 1-3 µg for 1 x 10^6 cells
    ICC/IF: 1-3 µg/mL
    Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freezing and thawing cycles.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Liew, Assmann, Templin, Raum, Lipson, Rajan, Qiang, Hu, Kawamori, Lindberg, Philipson, Sonenberg, Goldfine, Stoffers, Mirmira, Urano, Kulkarni: "Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 22, pp. E2319-28, (2014) (PubMed).

    Wang, Li, Felsovalyi, Chen, Cardozo, Krogsgaard: "Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers." in: ACS chemical biology, Vol. 9, Issue 9, pp. 2165-72, (2014) (PubMed).

    Kim, Li, Ahn, Song, Zhang: "Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity." in: Scientific reports, Vol. 4, pp. 7330, (2014) (PubMed).

    Van Gorp, Van Breedam, Van Doorsselaere, Delputte, Nauwynck: "Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus." in: Journal of virology, Vol. 84, Issue 6, pp. 3101-5, (2010) (PubMed).

  • Target
    V5 Epitope Tag
    Andere Bezeichnung
    V5 Tag (V5 Epitope Tag Produkte)
    Substanzklasse
    Tag
    Hintergrund
    The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
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