V5 Epitope Tag Antikörper

Details zu Produkt Nr. ABIN962847, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
399
18
16
3
3
Wirt
Maus
247
136
34
7
5
4
3
1
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
30
27
21
13
12
12
11
11
11
10
10
10
10
9
8
8
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
365
260
135
93
76
71
51
35
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke THE™
Immunogen V5 tag peptide conjugated - KLH
Sequenz GKPIPNPLLG LDST
Klon 4C12E11
Isotyp IgG2a
Spezifität THETM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Produktmerkmale THETM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
Reinigung Protein A affinity column
Andere Bezeichnung V5 Tag
Substanzklasse Tag
Hintergrund The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
Forschungsgebiet Tags/Labels
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL
Western blot: 0.2-1 µg/mL
Immunoprecipitation: 1-5 µg/mL
FACS: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Produkt verwendet in: Liew, Assmann, Templin, Raum, Lipson, Rajan, Qiang, Hu, Kawamori, Lindberg, Philipson, Sonenberg, Goldfine, Stoffers, Mirmira, Urano, Kulkarni: "Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 22, pp. E2319-28, 2014 (PubMed).

Wang, Li, Felsovalyi, Chen, Cardozo, Krogsgaard: "Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers." in: ACS chemical biology, Vol. 9, Issue 9, pp. 2165-72, 2014 (PubMed).

Kim, Li, Ahn, Song, Zhang: "Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity." in: Scientific reports, Vol. 4, pp. 7330, 2014 (PubMed).

Van Gorp, Van Breedam, Van Doorsselaere, Delputte, Nauwynck: "Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus." in: Journal of virology, Vol. 84, Issue 6, pp. 3101-5, 2010 (PubMed).

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