V5 Epitope Tag Antikörper (HRP)
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- Target Alle V5 Epitope Tag Produkte
- V5 Epitope Tag
- Reaktivität
- Bitte anfragen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser V5 Epitope Tag Antikörper ist konjugiert mit HRP
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Applikation
- Western Blotting (WB), ELISA
- Marke
- THE™
- Sequenz
- GKPIPNPLLG LDST
- Spezifität
- THETM V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins.
- Produktmerkmale
- The THETM V5 Tag Antibody [HRP], mAb, Mouse is THETM V5 Tag Antibody, mAb, Mouse conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
- Aufreinigung
- Protein A affinity column
- Immunogen
- V5 tag peptide conjugated - KLH
- Klon
- 4C12E11
- Isotyp
- IgG2a
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- Applikationshinweise
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL.Western blot: 0.2-1 µg/mL. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
- Buffer
- PBS, pH 7.4, containing 10 mg/mL BSA and 0.01 % Thimerosal.
- Konservierungsmittel
- Thimerosal (Merthiolate)
- Vorsichtsmaßnahmen
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing cycles.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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- Target
- V5 Epitope Tag
- Andere Bezeichnung
- V5 Tag (V5 Epitope Tag Produkte)
- Substanzklasse
- Tag
- Hintergrund
- V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
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