CD4 Molecule (CD4) Antikörper

Antigen: CD4 Molecule (CD4)

Synonyme: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Klonalität: Monoklonal (1F6)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN96243

Produktbeschreibung

Weitere Bezeichnung: CD4

Immunogen: Synthetic peptide corresponding to the external domain of CD4 molecule.

Format: Ig-fraction (prediluted)

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 1F6

Spezifität: This antibody reacts specifically with CD4 cells. CD4 is a single chain transmembrane glycoprotein with a molecular weight of 59 kD. The CD4 antigen is found on a T cell subset (helper/inducer) and also on thymocytes and monocytes.

Anwendungen

Applikationshinweise: Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking technique is required for paraffin embedded tissue sections. We recommend using 1mM EDTA pH 8.0 buffer instead of sodium citrate pH 6.0 buffer for antigen unmasking. Positive Control: Tonsil. Cellular localization: Cell membrane

Konzentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: CD Antigene

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Gay, Maddon, Sekaly et al.: "Functional interaction between human T-cell protein CD4 and the major histocompatibility complex HLA-DR antigen." in: Nature, Vol. 328, Issue 6131, pp. 626-9, 1987 (PubMed).

Dalgleish, Beverley, Clapham et al.: "The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus." in: Nature, Vol. 312, Issue 5996, pp. 763-7, 1985 (PubMed).

Stein, Lennert, Feller et al.: "Immunohistological analysis of human lymphoma: correlation of histological and immunological categories." in: Advances in cancer research, Vol. 42, pp. 67-147, 1985 (PubMed).