Bcl-X Antikörper

Antigen: Bcl-X

Klonalität: Monoklonal (2H12)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN95584

Produktbeschreibung

Immunogen: BALB/C mice were injected with a synthetic peptide of amino acid 3-14 (Cys-QSNRELVVDFLS) of human bcl-X protein.

Format: Supernatant

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 2H12

Spezifität: This antibody recognizes a 27 kD protein identified as bcl-XL protein. This antibody is highly specific to bcl-X and does not show cross reactivity with bcl-2 alpha or bax protein. This antibody cross reacts with human, mouse, rat and pig.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Hodgkin

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Krebs

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human Hodgkins lymphoma stained with bcl-X antibody

Publikationen

Publikationen: Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, 1997 (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed).

Wolter, Hsu, Smith et al.: "Movement of Bax from the cytosol to mitochondria during apoptosis." in: The Journal of cell biology, Vol. 139, Issue 5, pp. 1281-92, 1997 (PubMed).