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Antikörper
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anti-B-Cell CLL/lymphoma 2 (BCL2) Antikörper
B-Cell CLL/lymphoma 2 (BCL2) Antikörper
| Antigen |
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| Synonyme |
Bcl-2, bcl-2, BCL2 |
| Klonalität |
Monoklonal (124) |
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Wirt
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Reaktivität
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Human (180), Maus (76), Ratte (Rattus) (74), Hund (38), Rind (Kuh) (37), Schwein (35), Kaninchen (22), Huhn (18), Pferd (18), Schaf (3), Katze (2), Affe (2), Hamster (1)
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Applikation
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Western Blot (WB) (134), Immunfluoreszenz (IF) (74), Immunhistochemie (Paraffinschnitte) (IHC (p)) (57), Immunpräzipitation (IP) (56), Durchflusszytometrie (FACS) (50), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (40), Immunhistochemie (IHC) (39), ELISA (26), Enzyme Immunoassay (EIA) (21), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunzytochemie (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2), Intrazelluläre Durchflusszytometrie (ICFC) (2)
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| Produktnummer |
ABIN95568 |
| Menge |
6ml (1 to 4 ug/ml) (Varianten) |
| Preis |
191,35 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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Bcl-2
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Immunogen
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BALB/C mice were immunized with synthetic peptide sequence comprising amino acids 51-54 of bcl-2 protein.
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Format
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Supernatant (prediluted)
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Isotyp
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IgG1 kappa (Passende Sekundärantikörper)
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Klon
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124
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Spezifität
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This antibody reacts with a 25 kD bcl protein, which lies within the cell rather than on the cell surface. It stains neoplastic cells of follicular lymphoma, hairy cell leukemia, high grade B and T cell lymphomas, lymphoblastic lymphomas and anaplastic large cell lymphoma.
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Applikationshinweise
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Overfixation in formalin should be avoided, as it can give inconsistent results. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cell membrane
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Konzentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Forschungsgebiet
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Krebs
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Publikationen
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Hockenbery, Nuunez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).
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Alternativen zu Antigen "B-Cell CLL/lymphoma 2 (BCL2)", Typ "Antikörper" finden
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Wirte
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Kaninchen (142), Maus (49), Ziege (5), Schaf (4)
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Reaktivitäten
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Human (180), Maus (76), Ratte (Rattus) (74), Hund (38), Rind (Kuh) (37), Schwein (35), Kaninchen (22), Huhn (18), Pferd (18), Schaf (3), Katze (2), Affe (2), Hamster (1)
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Applikationen
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Western Blot (WB) (134), Immunfluoreszenz (IF) (74), Immunhistochemie (Paraffinschnitte) (IHC (p)) (57), Immunpräzipitation (IP) (56), Durchflusszytometrie (FACS) (50), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (40), Immunhistochemie (IHC) (39), ELISA (26), Enzyme Immunoassay (EIA) (21), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunzytochemie (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2), Intrazelluläre Durchflusszytometrie (ICFC) (2)
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Konjugate
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PE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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pThr56 (7), pSer70 (5), BH3 Domain Specific (1)
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Alternativen 
