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BCL2-Associated X Protein (BAX) Antikörper
| Antigen | BCL2-Associated X Protein (BAX) |
| Synonyme | BCL2L4, bax, fj16e01, wu:fc50b10, wu:fj16e01, BAX, bax-A, xBax, xlbax, MGC76049, BAX-ALPHA |
| Klonalität | Monoklonal (2D2) |
| Wirt |
 Alternativen Maus |
| Reaktivität |
Alternativen Human |
| Applikation |
Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p))
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3 Publikationen vorhanden |
| Produktnummer | ABIN95540 |
| Menge | 6ml (1 to 4 ug/ml) (Varianten) |
| Preis | 220,19 € Zzgl. Versandkosten €20,00 und MWSt |
| Lieferung nach |
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| Verfügbarkeit | Lieferung in 5 Werktagen |
Produktbeschreibung
| Weitere Bezeichnung | Bax |
| Immunogen | BALB/C mice were injected with a synthetic peptide amino acids 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein. |
| Format | Supernatant (prediluted) |
| Isotyp | IgG1 (Passende Sekundärantikörper) |
| Klon | 2D2 |
| Spezifität | This antibody recognizes a 211 kD protein identified as bax protein. This antibody is highly specific to bax and does not cross react with bcl-2 alpha or bcl-X protein. This antibody does not cross react with mouse and rat. |
Anwendungen
| Applikationshinweise | Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Lymphoma. Cellular localization: Cell membrane, cytoplasm |
| Konzentration | 1 to 4 ug/ml |
| Buffer | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. |
| Lagerung | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Forschungsgebiet | Apoptose/Nekrose |
| Beschränkungen | Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen. |
Publikationen
| Publikationen |
Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, 1997 (PubMed).
Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed). Wolter, Hsu, Smith et al.: "Movement of Bax from the cytosol to mitochondria during apoptosis." in: The Journal of cell biology, Vol. 139, Issue 5, pp. 1281-92, 1997 (PubMed). |
Alternativen
Alternativen zu Antigen "BCL2-Associated X Protein (BAX)", Typ "Antikörper" finden
| Wirte | Maus (103), Kaninchen (89), Schaf (3) |
| Reaktivitäten | Human (167), Maus (98), Ratte (Rattus) (91), Hund (33), Rind (Kuh) (32), Schwein (31), Schaf (30), Affe (3), Huhn (1), Pferd (1), Kaninchen (1) |
| Applikationen | Western Blot (WB) (132), Immunfluoreszenz (IF) (76), Immunpräzipitation (IP) (62), Immunhistochemie (Paraffinschnitte) (IHC (p)) (48), Durchflusszytometrie (FACS) (38), ELISA (18), Immunhistochemie (IHC) (17), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (15), Enzyme Immunoassay (EIA) (12), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2), Intrazelluläre Durchflusszytometrie (ICFC) (2) |
| Konjugate | Biotin (8), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2) |
| Epitope | N-Term (3), universal (1) |
