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Glycine Amidinotransferase (L-Arginine:glycine Amidinotransferase) (GATM) Antikörper

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Synonyme AT, AGAT, CCDS3, 1810003P21Rik, AI314789, cb409, wu:fa08a06, zgc:65855
Amino Acid
(77), (24), (23), (2), (1), (1), (1), (1), (1), (1)
(68), (12)
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC)
(62), (39), (26), (11), (9), (6), (5), (2), (2), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen Neu
Produkt-ID vom Hersteller Anmelden zum Anzeigen Neu
Menge 100 μL
Lieferung nach Deutschland ( )
Verfügbarkeit Lieferung in 2 bis 3 Werktagen
Immunogen Synthetic L-arginine conjugated to protein carrier (Pc)
Isotyp IgG
Spezifität Conjugated L-arginine . Using a conjugate L-arginine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compound Cross-reactivity ratio (a) Arginine-G-(Pc) 1 Glutamine-G-(Pc) 1/> 100,000 Asparagine-G-(Pc) 1/> 100,000 Lysine-G-(Pc) 1/> 100,000 (a): Arginine-G-(Pc) concentration / other conjugated aminoacids concentration at half displacement G = Glutaraldehyde
Reinigung Antiserum previously preabsorbed on protein carriers, and purified.
Andere Bezeichnung Arginine, L (GATM Antibody Abstract)
Substanzklasse Amino Acid
Forschungsgebiet Hormones, Receptors
Applikationshinweise Example of cytochemistry application Perfusion protocol for adult male sprague dawley (weight around 0.5kg):1- The animals can be deeply anesthetized with for example urethane (0.5-1.5g/kg, intraperitoneal). 2- Heparinized, and perfused via the ascending aorta with 100ml of cold physiologic saline (0.9% NaCl) and with the following fixative solution:a) 300ml of cold 4% paraformaldehyde and 2% glutaraldehyde in 0.1M phosphate-buffer (PB), pH 7.2 (two minutes). b) 600ml of cold 4% paraformaldehyde and 2% glutaraldehyde in 0.1M phosphate-buffer (PB), pH 7.2 (ten minutes). c) Dissect out the brains and place in a solution of 4% paraformaldehyde in 0.1M PB, pH 7.2, at 4ºC for twelve to sixteen hours. d) Before the brains will be cut on a freezing microtome, we must include the brain in growing concentrations of sucrose (a first bain of 5% of sucrose in PBS until the brains sank), after that we will repeat the same process in a solution with a higher level of sucrose (10%), 20%, 25% and finally 30%. Around 50 µm-thick serial sections will be obtained, kept at 4º C in PBS (0.1M, pH 7.2) and processed for immunostaining. Example of immunohistochemical protocol 1- In order to avoid possible interference with endogenous peroxidase, free-floating sections will be treated with distilled water containing NH 3 (20%), H 2 O 2 (30%) and NaOH (1%) for 20 min (other method is using a solution with 33% of H 2 O 2 and 66% of methanol). 2- Then, wash the sections for 20 min in 0.15M phosphate-buffered saline (PBS) (pH 7.2) 3- Pre-incubate for 30 min in PBS containing 10% of normal horse serum and 0.3% of Triton X-100 (mixed solution). 4- Incubate at room temperature (1h30min) and overnight at 4ºC in the same mixed solution containing L-Arginine antiserum (diluted as recommended dilution). 5- Then, the sections will be wash in PBS (30 min). 6- After that we will incubate for 60 min at room temperature with biotinylated anti-rabbit immunogammaglobulin (Vector) diluted 1/200 in PBS. 7- Wash during 30 min with PBS. 8- Sections will be incubated for 1h with a 1/100 diluted avidin-biotin-peroxidase complex (Vectastain). 9- After that we will wash the sections in PBS (30 min) 10- Wash with Tris-HCl buffer (pH 7.6)(10 min). 11- The tissue-bound peroxidase will be developed with H 2 O 2 using 3, 3'-diaminobenzidine as chromogen. 12- Finally the sections will be rinsed with PBS and coverslipped with PBS/Glycerol (1/1). Gemacbio sells the anti-conjugated Citrulline and anti-conjugated Agmatine antibodies raised in rabbit: used together, these tools could be helpful for immunocytochemistry double labelling. Recommended dilutions for Immunocytochemistry (1/1,000-1/5,000) Recommended dilutions for Western Blot (1/1,000-1/2,000)
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Lagerung 4 °C
Allgemeine Veröffentlichungen King, Stinus, le Moal et al.: "Central administration of arginine vasotocin: effects on exploratory behavior in the rat." in: Psychopharmacology, Vol. 76, Issue 1, pp. 40-3, 1982 (PubMed).

King, Geffard, Chavreau et al.: "Des-tyr-gamma-endorphin (DT gamma E) and pineal levels of melatonin and arginine vasopressin (AVP)." in: European journal of pharmacology, Vol. 76, Issue 2-3, pp. 271-4, 1982 (PubMed).

Produktnummer ABIN95359
531,14 €
Zzgl. Versandkosten 20,00 € und MWSt

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