CDT2/RAMP Antikörper (Middle Region)

Produktnummer ABIN951451

Produktdetails anti-CDT2/RAMP Antikörper

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Bindungsspezifität: AA 229-256, Middle Region

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDT2/RAMP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Spezifität: This antibody recognizes Human and Mouse CDT2 / DTL (Center).

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of Human DTL.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CDT2/RAMP Antikörper (ABIN951451)

Abbas, Sivaprasad, Terai, Amador, Pagano, Dutta: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, (2008) (PubMed).

Details zu CDT2/RAMP

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Andere Bezeichnung: CDT2 / DTL (DTL Produkte)

Synonyme: CDT2 antikoerper, DCAF2 antikoerper, L2DTL antikoerper, RAMP antikoerper, 2810047L02Rik antikoerper, 5730564G15Rik antikoerper, Ramp antikoerper, RGD1310439 antikoerper, cdt2 antikoerper, cdt2-a antikoerper, dcaf2 antikoerper, l2dtl antikoerper, ramp antikoerper, cb151 antikoerper, chunp6871 antikoerper, wu:fb54b02 antikoerper, denticleless E3 ubiquitin protein ligase homolog antikoerper, denticleless E3 ubiquitin protein ligase antikoerper, denticleless E3 ubiquitin protein ligase homolog S homeolog antikoerper, denticleless E3 ubiquitin protein ligase homolog (Drosophila) antikoerper, DTL antikoerper, Dtl antikoerper, dtl.S antikoerper, dtl antikoerper

Hintergrund: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.Synonyms: CDW1, DCAF2, Ddb1- and Cul4-associated factor 2, Denticleless protein homolog, L2DTL, Lethal(2) denticleless protein homolog, RA regulated Nuclear Matrix Associated Protein, RAMP, Retinoic acid-regulated nuclear matrix-associated protein

Molekulargewicht: 79468 Da

Gen-ID: 51514

NCBI Accession: NP_057532