Actinin Antikörper

Antigen: Actinin

Klonalität: Monoklonal (EA-53)

Wirt: Maus

Reaktivität: Human, Säugetiere

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN94616

Produktbeschreibung

Immunogen: Purified rabbit skeletal alpha-actinin.

Format: Ascites

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: EA-53

Spezifität: This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non- sarcomeric muscle elements. This antibody shows wide range of cross reactivity with various mammalian species.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Skeletal muscle. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Goncharova, Kam, Geiger: "The involvement of adherens junction components in myofibrillogenesis in cultured cardiac myocytes." in: Development (Cambridge, England), Vol. 114, Issue 1, pp. 173-83, 1992 (PubMed).