Linker For Activation of T Cells (LAT) Antikörper

Antigen: Linker For Activation of T Cells (LAT)

Synonyme: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Klonalität: Monoklonal (LAT-1D1)

Wirt: Maus

Reaktivität: Maus, Ratte (Rattus)

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunzytochemie (ICC)

Produktnummer: ABIN94420

Produktbeschreibung

Weitere Bezeichnung: LAT

Immunogen: Bacterially expressed full-length rat LAT

Format: Purified

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: LAT-1D1

Beschreibung: LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembraneadaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Afterimmunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, orTec-family kinases, providing docking sites for downstream signaling molecules. LAT isessential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as wellas for maturation of early thymocytes. It is also involved in NK cell signaling and plateletactivation.

Spezifität: The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), atransmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells andplatelets. This antibody does not react with: Human.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 5-10 µg/ml. Positive control: mouse spleen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Signaltransduktion, Adaptive Immunität

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC, 1A) and RBL rat basophilic leukemia cell line (1B) by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody (cat. no. 11-487-C100), followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.

Publikationen

Publikationen: Tolar P, Tumova M, Draber P: "New monoclonal antibodies recognizing the adaptor protein LAT." in: Folia Biol (Praha), Vol. 47, Issue 6, pp. 215-7, 2007 (PubMed).