HLA Class I Antikörper

Antigen: HLA Class I

Klonalität: Monoklonal (W6-32)

Wirt: Maus

Reaktivität: Human, Primaten, Rind (Kuh), Katze

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Funktionale Studien (Func), ELISA, Immunzytochemie (ICC)

Produktnummer: ABIN94332

Produktbeschreibung

Weitere Bezeichnung: HLA-Class I

Immunogen: Membrane of human tonsil cells

Format: Azide Free

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: W6-32

Beschreibung: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta2microglobulin. They carry foreign determinants important for immune recognition bycytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class Iantigens are represented by HLA-A, HLA-B and HLA-C molecules.

Spezifität: The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressedon the surface of all human nucleated cell types.The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expressionin different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathyand other neuromuscular disorders.This antibody W6/32 is also suitable as a positive control for HLA tissue typing andcrossmatching. This antibody does not react with: Rabbit.

Anwendungen

Applikationshinweise: Western Blotting. Application note: Non-reducing conditions. Functional Application: The antibody W6/32 is suitable as a positive control for HLA tissue typing.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein G-affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Haupthistokompatibilitätskomplex (MHC)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Surface staining of human peripheral blood cells with anti-HLA-class I (W6/32) Alexa Fluor® 488. 

Publikationen

Publikationen: Gonzalez, Alegre, Torres et al.: "Evaluation of HLA-G5 Plasmatic Levels During Pregnancy and Relationship with the 14-bp Polymorphism." in: American journal of reproductive immunology (New York, N.Y. : 1989), 2010 (PubMed).