PAG / CBP Antikörper (Dyomics 647)

Antigen: PAG / CBP

Klonalität: Monoklonal (MEM-255)

Wirt: Maus

Reaktivität: Human

Konjugat: Dyomics 647

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN94273

Produktbeschreibung

Immunogen: Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Isotyp: IgG2a

Klon: MEM-255

Beschreibung: PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is aubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts(glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gelsanomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src familykinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the majornegative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells orhigh affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosinephosphorylation and Csk binding, while T cell receptor signaling causes PAGdephosphorylation, loss of Csk binding and increased activation of the protein tyrosinekinase Lck.

Spezifität: The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp)located in the cytoplasmic domain, also known as protein associated withglycosphingolipid-enriched microdomains (PAG). This antibody does not react with: Mouse, Rat, Bovine .

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 1000.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Konzentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Preparation: The purified antibody is conjugated with Dyomics 647 (DY647) under optimum conditions.The conjugate is purified by size-exclusion chromatography..

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemistry of paraffin-embedded sections