CD95 Antikörper
| Antigen |
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| Klonalität |
Monoklonal (UT-1) |
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Wirt
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Reaktivität
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Human (182), Maus (18), Ratte (Rattus) (9), Affe (7), Katze (3), Hund (3), Rind (Kuh) (1), Fruchtfliege (Drosophila melanogaster) (1), Hamster (1), Schwein (1), Kaninchen (1), Schaf (1), Xenopus laevis (1)
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Funktionale Studien (Func)
Durchflusszytometrie (FACS) (126), Western Blot (WB) (61), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (42), Immunpräzipitation (IP) (42), Enzyme Immunoassay (EIA) (31), Immunhistochemie (Paraffinschnitte) (IHC (p)) (26), Funktionale Studien (Func) (14), Immunfluoreszenz (IF) (12), Blockierende Antikörper (Inhibition) (8), ELISA (7), Apoptose-Induktion (AI) (4), Immunhistochemie (IHC) (4), Dot Blot (Dot) (3), ELISA (Detektionsantikörper) (3), Neutralisierung (Neut) (1)
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| Produktnummer |
ABIN94250 |
| Menge |
0.1 mg (1 mg/ml) |
| Preis |
230,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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CD95 / Fas
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Immunogen
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HUT-78 human T cell lymphoma cells
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Format
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Azide Free
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Isotyp
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IgM (Passende Sekundärantikörper)
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Klon
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UT-1
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Beschreibung
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CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of theTNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular deathdomains, formation of the death-inducing signaling complex (DISC) and activation ofcaspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated atthe DISC, in type II cells low concentration of caspase 8 activates pathway leading to therelease of cytochrome c from mitochondria and activation of caspase 3 by cytochom c.Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokineresponses.
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Spezifität
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The antibody UT-1 reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumournecrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a varietyof normal and neoplastic cells.The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11antibody.
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Applikationshinweise
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Functional Application: The antibody UT-1 induces Fas-mediated apoptosis. Flow Cytometry
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from ascites by precipitation methods and ion exchange chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.
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Lagerung
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "CD95", Typ "Antikörper" finden
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Wirte
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Maus (154), Kaninchen (34), Ratte (6), Huhn (2), Ziege (1)
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Reaktivitäten
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Human (182), Maus (18), Ratte (Rattus) (9), Affe (7), Katze (3), Hund (3), Rind (Kuh) (1), Fruchtfliege (Drosophila melanogaster) (1), Hamster (1), Schwein (1), Kaninchen (1), Schaf (1), Xenopus laevis (1)
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Applikationen
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Durchflusszytometrie (FACS) (126), Western Blot (WB) (61), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (42), Immunpräzipitation (IP) (42), Enzyme Immunoassay (EIA) (31), Immunhistochemie (Paraffinschnitte) (IHC (p)) (26), Funktionale Studien (Func) (14), Immunfluoreszenz (IF) (12), Blockierende Antikörper (Inhibition) (8), ELISA (7), Apoptose-Induktion (AI) (4), Immunhistochemie (IHC) (4), Dot Blot (Dot) (3), ELISA (Detektionsantikörper) (3), Neutralisierung (Neut) (1)
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Konjugate
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FITC (21), PE (18), APC (8), Biotin (8), Alexa Fluor 488 (2), SPRD (2), Alexa Fluor 647 (1), PE,Cy5 (1)
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Epitope
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C-Term (4), pTyr232 (2), pTyr291 (2), Center (1)
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