CD33 Molecule (CD33) Antikörper (APC)

Antigen: CD33 Molecule (CD33)

Synonyme: CD33, p67, SIGLEC3, FLJ00391, SIGLEC-3

Klonalität: Monoklonal (HIM3-4)

Wirt: Maus

Reaktivität: Human, Primaten

Konjugat: APC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN94075

Produktbeschreibung

Weitere Bezeichnung: CD33

Immunogen: NFMY-9s human cell line

Isotyp: IgG1

Klon: HIM3-4

Beschreibung: CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin(Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif(ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 andSHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of thereceptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, bindssialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target foracute myeloid leukemia.

Spezifität: The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein(immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes,dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes andhematopoietic stem cells.HLDA V, WS Code M MA112HLDA VI, WS Code M MA47

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Bonnet: "Humanized model to study leukemic stem cells." in: Methods in molecular biology (Clifton, N.J.), Vol. 538, pp. 247-62, 2009 (PubMed).

Hegde, Jankowska-Gan, Roenneburg et al.: "Human NKT cells promote monocyte differentiation into suppressive myeloid antigen-presenting cells." in: Journal of leukocyte biology, Vol. 86, Issue 4, pp. 757-68, 2009 (PubMed).