Selectin P Ligand (SELPLG) Antikörper

Antigen: Selectin P Ligand (SELPLG)

Synonyme: CLA, CD162, PSGL1, PSGL-1, SELPLG, SELPG

Klonalität: Monoklonal (TB5)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN93998

Produktbeschreibung

Weitere Bezeichnung: CD162

Format: Purified

Isotyp: IgG1

Klon: TB5

Beschreibung: CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressedas a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulatingleukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity forP-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite forfirm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediatesleukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhessionmolecule on mature leukocytes, CD162 is a potent negative regulator of humanhematopoietic progenitors.

Spezifität: The antibody TB5 reacts with CD162, a 220 kDa type I integral membrane proteinexpressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the mostperipheral blood T lymphocytes, monocytes, granulocytes, it is also expressed on asubpopulation of B lymphocytes and CD34 + bone marrow cells.

Anwendungen

Applikationshinweise: Immunohistochemistry (paraffin sections) - Positive tissue: tonsil, spleen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Kardiovaskuläres System, Artherosklerose, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Publikationen

Publikationen: Marathe, Chandrasekaran, Lau et al.: "Systems-level studies of glycosyltransferase gene expression and enzyme activity that are associated with the selectin binding function of human leukocytes." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 12, pp. 4154-67, 2008 (PubMed).