CD10 Antikörper

Antigen: CD10

Klonalität: Monoklonal (MEM-78)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP)

Produktnummer: ABIN93930

Produktbeschreibung

Gen-ID: 3q25.1-q25.2

Immunogen: NALM-6 human pre-B cell line

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MEM-78

Beschreibung: CD10 (neutral endopeptidase NEP, common acute lymphocytic leukemia antigen CALLA,membrane metallo-endopeptidase MME, enkefalinase) is a 100-kDa cell surface zincmetalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids andinactivating multiple physiologically active peptids. CD10 is expressed on various normal celltypes, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelialcells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiationpathway and has a key role in regulation of growth, differentiation and signal transduction ofmany cellular systems.

Spezifität: The antibody MEM-78 reacts with CD10 antigen (CALLA - Common acute lymphaticleukemia antigen), a 100 kDa type II integral membrane protein.HLDA IV, WS Code B 506HLDA V, WS Code B CD10.4

Anwendungen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Krebs, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Adaptive Immunität, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Angelisovua, Drbal, Horejsui et al.: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).