Tubulin, beta (TUBB) Antikörper

Antigen: Tubulin, beta (TUBB)

Synonyme: M40, TUBB1, TUBB5, MGC16435, MGC117247, OK/SW-cl.56, m40, XLOT, tubb1, tubb5, MGC69508, ok/sw-cl.56, TUBB, TUBB2A, MGC128415, 1t, B1t, BETA 56D, DTB2, Dmbeta1, T, Tub, Tubulin, beta-Tub56D, beta-tub, beta-tubulin56D, beta1, beta1-Tubulin, beta1-tub, beta1Tub, beta1t, beta56D, betaTub1, beta[[1]] tubulin, beta[[1]]-tubulin, DmelCG9277, CG9277, 143391_i_at, 3t, B3t, BETA 60D, D.m.BETA-60D, DTB3, Dmbeta3, beta-Tub60D, beta-Tub6D, beta3, beta3 TU, beta3-Tub, beta3t, beta60C, betaTub3, betaTub60C, beta[[3]] tubulin, beta[[3]]-Tub, beta[[3]]-tubulin, p50, p50/tubulin, p53, p53/tubulin, DmelCG3401, CG3401, 2t, B2t, BETA 85D, BETA2, D.m.BETA-85D, DTB4, Dmbeta2, beta(2)Tu, beta(2)Tub, beta-Tub85D, beta2t, beta2tub, beta85D, betaTub2, beta[[2]]-tubulin, ms(3)KK[D], DmelCG9359, CG9359, 4t, B4t, BETA 98B, beta-Tub, beta-Tub97EF, beta4t, beta97F, betaTub4, betaTub98BC, betaTub98C, CG4869, Dmbeta4, DmelCG4869, DTB1, tubB3, tubulin, Tub1, bmtub1, Tub3, bmtub3, Tub2, bmtub2, Tub4, bmtub4, LOC100101153, TUBB2, dJ40E16.7, MC1R, TUBB4, beta-4, Tubb2, M(beta)2, beta-5, tubb2a, MGC69524, TUBB2C, MGC128183, TUBB3, MGC134115

Klonalität: Monoklonal (TU-11)

Wirt: Maus

Reaktivität: Schwein, Maus

Applikation: Western Blot (WB)

Produktnummer: ABIN93917

Produktbeschreibung

Weitere Bezeichnung: beta-tubulin

Immunogen: microtubule proteins from porcine brain

Format: Purified

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: TU-11

Beschreibung: The microtubules are intracellular dynamic polymers made up of evolutionarily conservedpolymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associatedproteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamicminus ends. Microtubules are required for vital processes in eukaryotic cells includingmitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are alsonecessary for movement of cells by means of flagella and cilia. In mammalian tissue culturecells microtubules have their minus ends anchored in microtubule organizing centers(MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimerto associate with other heterodimers to form microtubule. In vivo, microtubule dynamics varyconsiderably. Microtubule polymerization is reversible and a populations of microtubules incells are on their minus ends either growing or shortening this phenomenon is calleddynamic instability of microtubules. On a practical level, microtubules can easily bestabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or morecommonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at roomtemperature for many hours. Using limited proteolysis by enzymes both tubulin subunits canbe divided into N-terminal and C-terminal structural domains.The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulinin tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationallymodified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

Spezifität: The antibody TU-11 recognizes an epitope located on C-terminal domain of beta-tubulin invarious species.

Synonyme: M40, TUBB1, TUBB5, MGC16435, MGC117247, OK/SW-cl.56, m40, XLOT, tubb1, tubb5, MGC69508, ok/sw-cl.56, TUBB, TUBB2A, MGC128415, 1t, B1t, BETA 56D, DTB2, Dmbeta1, T, Tub, Tubulin, beta-Tub56D, beta-tub, beta-tubulin56D, beta1, beta1-Tubulin, beta1-tub, beta1Tub, beta1t, beta56D, betaTub1, beta[[1]] tubulin, beta[[1]]-tubulin, DmelCG9277, CG9277, 143391_i_at, 3t, B3t, BETA 60D, D.m.BETA-60D, DTB3, Dmbeta3, beta-Tub60D, beta-Tub6D, beta3, beta3 TU, beta3-Tub, beta3t, beta60C, betaTub3, betaTub60C, beta[[3]] tubulin, beta[[3]]-Tub, beta[[3]]-tubulin, p50, p50/tubulin, p53, p53/tubulin, DmelCG3401, CG3401, 2t, B2t, BETA 85D, BETA2, D.m.BETA-85D, DTB4, Dmbeta2, beta(2)Tu, beta(2)Tub, beta-Tub85D, beta2t, beta2tub, beta85D, betaTub2, beta[[2]]-tubulin, ms(3)KK[D], DmelCG9359, CG9359, 4t, B4t, BETA 98B, beta-Tub, beta-Tub97EF, beta4t, beta97F, betaTub4, betaTub98BC, betaTub98C, CG4869, Dmbeta4, DmelCG4869, DTB1, tubB3, tubulin, Tub1, bmtub1, Tub3, bmtub3, Tub2, bmtub2, Tub4, bmtub4, LOC100101153, TUBB2, dJ40E16.7, MC1R, TUBB4, beta-4, Tubb2, M(beta)2, beta-5, tubb2a, MGC69524, TUBB2C, MGC128183, TUBB3, MGC134115

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 2 µg/ml, 60 min in room temperature Positive control: Porcine brain lysate. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 3min in water bath. Application note: Reducing conditions.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by precipitation methods and size-exclusion chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Draber, Draberova, Linhartova et al.: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." in: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, 1990 (PubMed).