ATP1B1 Antikörper (Subunit beta)

Produktnummer ABIN5684017

Produktdetails anti-ATP1B1 Antikörper

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Bindungsspezifität: Subunit beta

Reaktivität: Ratte, Arabidopsis thaliana, Schwein, Spinach, Zea mays subsp. parviglumis (Balsas teosinte), Soybean, Salmon, Oryza sativa, Nicotiana tabacum, Nicotiana benthamiana, Chlamydomonas reinhardtii, Huhn, Gerste, Bacillus cereus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP1B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Blue-native PAGE (BN PAGE)

Spezifität: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases. Peptide used for antibody production is located ina beta sheet, which is partly exposed near the surface of the AtpB protein.

Kreuzreaktivität (Details): No cross-reactivity with: archeal V-type ATP synthase

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 53.9kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 2000 - 1 : 5 000 with standard ECL (WB), 1 : 5000 (BN-PAGE).

Kommentare:

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 10 µL of sterile water

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-ATP1B1 Antikörper (ABIN5684017)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Berto, DAdamo, Bergantino, Vallese, Giacometti, Costantini: "The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins." in: Biochemical and biophysical research communications, (2011) (PubMed).

Ahsan, Nakamura, Komatsu: "Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress." in: Amino acids, (2010) (PubMed).

Details zu ATP1B1

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Andere Bezeichnung: AtpB (ATP1B1 Produkte)

Synonyme: ATP1B antikoerper, Atp4b antikoerper, Atpb antikoerper, Atpb-1 antikoerper, NKbeta1 antikoerper, ATPBS antikoerper, atp1b1 antikoerper, atp1b1a antikoerper, cb710 antikoerper, ATPase Na+/K+ transporting subunit beta 1 antikoerper, ATPase, Na+/K+ transporting, beta 1 polypeptide antikoerper, ATPase beta chain antikoerper, ATP synthase CF1 beta subunit antikoerper, ATPase Na+/K+ transporting subunit beta 1 S homeolog antikoerper, ATPase, Na+/K+ transporting, beta 1b polypeptide antikoerper, ATP1B1 antikoerper, Atp1b1 antikoerper, atpB antikoerper, atp1b1.S antikoerper, atp1b1b antikoerper

Hintergrund: AGI Code: At5g08670, ATCG00480
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

Molekulargewicht: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

UniProt: P83483, A8IQU3, P19366, P06541

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interaktom