psi-C Core Subunit of Photosystem I Antikörper

Antigen: psi-C Core Subunit of Photosystem I

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Barley (Hordeum vulgare), Spinat (Spinacia oleracea), Synechococcus PCC 7942, Cyanophora paradoxa, Heterosigma akashiwo, Thalassiosira pseudonana, Euglena gracilis, Micromonas pusilla, Chlamydomonas reinhardtii (C. reinhardtii), Red algae (Porphyra), Gonyaulax polyedra, Emiliania huxleyi

Applikation: Western Blot (WB)

Produktnummer: ABIN93546

Produktbeschreibung

Weitere Bezeichnung: PSI-C core subunit of photosystem I (PsaC)

Immunogen: KLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativaP0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatusQ31QV2

Format: Lyophilized

Beschreibung: PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately10kDa, present in all known Photosystem I complexes. It is located on the stromalside of the thylacoid membranes. PsaC coordinates the Fe–S clusters FA and FBthrough two cysteine-rich domains.

Spezifität: Predicted reactivity: dicots including Glycine max, Nicotiana tabacum, Spinacia oleracea, andmonocots, Physcomitrella patens, algae and cyanobacteria, Prochlorococcus sp.(surface and a deep water ecotype).

Anwendungen

Applikationshinweise: Recommended Dilution 1: 10 000 with ECL (WB). Additional Information: Peptide target used to elicit this antibody is well conserved in all photoautotrophsexcept some cyanobacteria, some red algae and Cyanophora paradoxa, whichcontain a conserved substitution of a valine to an isoleucine. The performance ofthe antibodies has been confirmed against taxa containing both the valine andisoleucine variants.

Buffer: Affinity purified serum in PBS pH 7.4

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of PsaC protein (10 kDa). Lane 1 - Markers 20, 30, 40, 50, 60, 80, 100, 120, 220 kDa (Magic Marks, Invitrogen).Lane 2 - Synechococcus PCC 7942 extractLane 3 - Spinach extractLane 4 - Heterosigma extract Anti-PsaC antibodies were used at 1 -50 0

Following lanes contained whole cell extracts from -1. Higher plants - Spinach, 2. Cyanobacteria - Synechococcus PCC 7942,3. Glaucocystophyte - Cyanophora paradoxa,4. Ochrophyte - Raphidophyte - Heterosigma akashiwo,5. Ochrophyte - Diatom - Thalassiosira

Publikationen

Publikationen: Kuepper, Setlik, Seibert et al.: "Iron limitation in the marine cyanobacterium Trichodesmium reveals new insights into regulation of photosynthesis and nitrogen fixation." in: The New phytologist, Vol. 179, Issue 3, pp. 784-98, 2008 (PubMed).

Munekage, Eymery, Rumeau et al.: "Elevated expression of PGR5 and NDH-H in bundle sheath chloroplasts in C4 flaveria species." in: Plant & cell physiology, Vol. 51, Issue 4, pp. 664-8, 2010 (PubMed).