Lhcii Type I Chlorophyll A/B-Binding Protein (Lhcb1) Antikörper

Antigen: Lhcii Type I Chlorophyll A/B-Binding Protein (Lhcb1)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Peanut (Arachis hypogaea), Barley (Hordeum vulgare), Tomate (Solanum lycopersicum), Mesembryanthemum crystallinum, Tabak (Nicotiana tabacum), Reis (Oryza sativa), Erbse (Pisum sativum), Spinat (Spinacia oleracea), Maize/Corn (Zea mays), Chlamydomonas reinhardtii (C. reinhardtii)

Applikation: Western Blot (WB), Immunhistochemie (IHC), Immunolokalisation (IL)

Produktnummer: ABIN93510

Produktbeschreibung

Immunogen: BSA-conjugated synthetic peptide derived from a highly conserved sequence ofLhb1 proteins from angiosperms (monocots and dicots) and gymnosperms,including Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2),At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420(Lhcb1.5)

Format: Lyophilized

Beschreibung: The major light-harvesting antenna complex II (LHCII) in photosyntheticeukaryotes is located in the thylakoid membrane of the chloroplast. It is aheterotrimeric complex formed by up to 3 different individual subtypes ofchlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the mostabundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often iscoded by several nuclear genes.

Spezifität: Reactivities tested in Western Blotting. Predicted reactivity: angiosperms (monocots and dicots), gymnosperms, mosses, green algae.

Anwendungen

Applikationshinweise: Recommended Dilution 1 : 2000, detected with standard ECL (WB), 1: 100 - 1: 500 (IG). Additional Information: A molecular characterisation of the LHCII proteins can be found in Caffarri et al.(2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 ComplexesBuilding the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis.Biochemistry 43 (29): 9467–9476 .

Buffer: Total IgG in PBS pH 7.4, lyophilized

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Yoshida, Watanabe, Kato et al.: "Influence of chloroplastic photo-oxidative stress on mitochondrial alternative oxidase capacity and respiratory properties: a case study with Arabidopsis yellow variegated 2." in: Plant & cell physiology, Vol. 49, Issue 4, pp. 592-603, 2008 (PubMed).

Tikkanen, Nurmi, Suorsa et al.: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, 2008 (PubMed).

Munekage, Eymery, Rumeau et al.: "Elevated expression of PGR5 and NDH-H in bundle sheath chloroplasts in C4 flaveria species." in: Plant & cell physiology, Vol. 51, Issue 4, pp. 664-8, 2010 (PubMed).

Yin, Lundin, Bertrand et al.: "Role of Thylakoid ATP/ADP Carrier in Photoinhibition and Photoprotection of Photosystem II in Arabidopsis." in: Plant physiology, 2010 (PubMed).

Yuan, Xu, Yuan et al.: "Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet." in: Journal of integrative plant biology, Vol. 52, Issue 9, pp. 809-16, 2010 (PubMed).

Saito, Iino, Sonoike et al.: "Remodeling of the major light-harvesting antenna protein of PSII protects the young leaves of barley (Hordeum vulgare L.) from photoinhibition under prolonged iron deficiency." in: Plant & cell physiology, Vol. 51, Issue 12, pp. 2013-30, 2010 (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, 2011 (PubMed).