TLR4 Antikörper (AA 751-835) (Alexa Fluor 488)

Produktnummer ABIN917904

Produktdetails anti-TLR4 Antikörper (Alexa Fluor 488)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Bindungsspezifität: AA 751-835

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TLR4 Antikörper ist konjugiert mit Alexa Fluor 488

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Homologie: Dog,Sheep,Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat TLR4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-TLR4 Antikörper (Alexa Fluor 488) (ABIN917904)

Takanashi, Tomosada, Villena, Murata, Takahashi, Chiba, Tohno, Shimazu, Aso, Suda, Ikegami, Itoh, Kawai, Saito, Alvarez, Kitazawa: "Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation." in: BMC microbiology, Vol. 13, pp. 54, (2013) (PubMed).

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: TLR4 (TLR4 Produkte)

Synonyme: ARMD10 antikoerper, CD284 antikoerper, TLR-4 antikoerper, TOLL antikoerper, Lps antikoerper, Ly87 antikoerper, Ran/M1 antikoerper, Rasl2-8 antikoerper, toll like receptor 4 antikoerper, toll-like receptor 4 antikoerper, TLR4 antikoerper, Tlr4 antikoerper

Hintergrund:

Synonyms: Lps, Ly87, Ran/M1, Rasl2-8, Toll-like receptor 4, CD284, Tlr4

Background: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.

Gen-ID: 21898

UniProt: Q9QX05

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine