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SLC12A1 Antikörper (AA 33-55)

SLC12A1 Reaktivität: Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN863200
  • Target Alle SLC12A1 Antikörper anzeigen
    SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))
    Bindungsspezifität
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 33-55
    Reaktivität
    • 26
    • 25
    • 14
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Ratte
    Wirt
    • 37
    • 2
    Kaninchen
    Klonalität
    • 35
    • 4
    Polyklonal
    Konjugat
    • 21
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser SLC12A1 Antikörper ist unkonjugiert
    Applikation
    • 32
    • 24
    • 10
    • 5
    • 5
    • 3
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Spezifität
    Detects ~160 kDa.
    Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Protein A Purified
    Immunogen
    Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
    Top Product
    Discover our top product SLC12A1 Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:25)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN863200 was sufficient for detection of NKCC2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Hyndman, Mironova, Giani, Dugas, Collins, McDonough, Stockand, Pollock: "Collecting Duct Nitric Oxide Synthase 1ß Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotensin II Pathways." in: Journal of the American Heart Association, Vol. 6, Issue 10, (2018) (PubMed).

    Limmer, Schinner, Castrop, Vitzthum, Hofmann, Schlossmann: "Regulation of the Na(+) -K(+) -2Cl(-) cotransporter by cGMP/cGMP-dependent protein kinase I after furosemide administration." in: The FEBS journal, (2015) (PubMed).

    Zhang, Sun, Ding, Huang, Zhang, Jia: "Inhibition of Mitochondrial Complex-1 Prevents the Downregulation of NKCC2 and ENaCα in Obstructive Kidney Disease." in: Scientific reports, Vol. 5, pp. 12480, (2015) (PubMed).

    Davies, Fraser, Galic, Choy, Katerelos, Gleich, Kemp, Mount, Power: "Novel mechanisms of Na+ retention in obesity: phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK." in: American journal of physiology. Renal physiology, Vol. 307, Issue 1, pp. F96-F106, (2014) (PubMed).

  • Target
    SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))
    Andere Bezeichnung
    NKCC2 (SLC12A1 Produkte)
    Synonyme
    slc12a1 antikoerper, SLC12A1 antikoerper, DKFZp469A2020 antikoerper, BSC1 antikoerper, NKCC2 antikoerper, Nkcc2 antikoerper, AI788571 antikoerper, D630042G03Rik antikoerper, mBSC1 antikoerper, urehr3 antikoerper, si:ch211-220f12.1 antikoerper, solute carrier family 12 member 1 antikoerper, solute carrier family 12, member 1 antikoerper, si:ch211-220f12.1 antikoerper, SLC12A1 antikoerper, Slc12a1 antikoerper
    Hintergrund
    NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
    Gen-ID
    25065
    NCBI Accession
    NP_001257546
    UniProt
    Q5PQV1
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