HIF1A Antikörper (AA 329-530)
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- Target Alle HIF1A Antikörper anzeigen
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
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Bindungsspezifität
- AA 329-530
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Reaktivität
- Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser HIF1A Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- Detects ~116 kDa. Specific for HIF1Alpha.
- Kreuzreaktivität
- Rind (Kuh), Human, Maus, Ratte
- Aufreinigung
- Protein G Purified
- Immunogen
- Recombinant fragment corresponding to amino acids 329-530
- Klon
- ESEE122
- Isotyp
- IgG1
- Top Product
- Discover our top product HIF1A Primärantikörper
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- Applikationshinweise
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:50)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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1 μg/ml of ABIN863096 was sufficient for detection of HIF1α in 20 μg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- -20°C
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The effect of estrogen on prolidase-dependent regulation of HIF-1? expression in breast cancer cells." in: Molecular and cellular biochemistry, Vol. 379, Issue 1-2, pp. 29-36, (2013) (PubMed).
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The effect of estrogen on prolidase-dependent regulation of HIF-1? expression in breast cancer cells." in: Molecular and cellular biochemistry, Vol. 379, Issue 1-2, pp. 29-36, (2013) (PubMed).
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- Target
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- Andere Bezeichnung
- HIF 1 alpha (HIF1A Produkte)
- Synonyme
- hif1a antikoerper, AA959795 antikoerper, HIF1alpha antikoerper, MOP1 antikoerper, bHLHe78 antikoerper, HIF-1A antikoerper, HIF-1alpha antikoerper, HIF1 antikoerper, HIF1-ALPHA antikoerper, PASD8 antikoerper, HIF1-alpha antikoerper, hif-1a antikoerper, hypoxia inducible factor 1 alpha antikoerper, hypoxia inducible factor 1, alpha subunit antikoerper, hypoxia inducible factor 1 alpha subunit antikoerper, HIF (Hypoxia Inducible Factor) homologa antikoerper, hif-1a antikoerper, Hif1a antikoerper, HIF1A antikoerper, hif1a antikoerper, hif-1 antikoerper
- Hintergrund
- Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
- Gen-ID
- 15251
- NCBI Accession
- NP_034561
- UniProt
- Q61221
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effekt
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