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Rhodopsin Antikörper

RHO Reaktivität: Rind (Kuh) WB, IHC, ELISA, IP, ICC, IF Wirt: Maus Monoclonal 1D4 unconjugated
Produktnummer ABIN863085
  • Target Alle Rhodopsin (RHO) Antikörper anzeigen
    Rhodopsin (RHO)
    Reaktivität
    • 39
    • 17
    • 16
    • 14
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Rind (Kuh)
    Wirt
    • 39
    • 25
    • 2
    • 1
    • 1
    Maus
    Klonalität
    • 35
    • 32
    • 1
    Monoklonal
    Konjugat
    • 27
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser Rhodopsin Antikörper ist unkonjugiert
    Applikation
    • 51
    • 41
    • 34
    • 24
    • 13
    • 11
    • 10
    • 8
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Spezifität
    Detects ~40 kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
    Kreuzreaktivität
    Vertebrate
    Aufreinigung
    Protein G Purified
    Immunogen
    Bovine Rhodopsin
    Klon
    1D4
    Isotyp
    IgG1
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN863084 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Makowka, Bruegmann, Dusend, Malan, Beiert, Hesse, Fleischmann, Sasse: "Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision." in: Nature communications, Vol. 10, Issue 1, pp. 1281, (2019) (PubMed).

    Cheng, Djajadi, Molday: "Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy." in: Methods in molecular biology (Clifton, N.J.), Vol. 935, pp. 185-99, (2012) (PubMed).

  • Target
    Rhodopsin (RHO)
    Andere Bezeichnung
    Rhodopsin (RHO Produkte)
    Synonyme
    CSNBAD1 antikoerper, OPN2 antikoerper, RP4 antikoerper, RH1 antikoerper, Rh antikoerper, fi06d11 antikoerper, wu:fi06d11 antikoerper, zfo2 antikoerper, zfrho antikoerper, Noerg1 antikoerper, Opn2 antikoerper, Ops antikoerper, opn2 antikoerper, rh1 antikoerper, rp4 antikoerper, xrho antikoerper, PIGRHO1 antikoerper, ops antikoerper, RDP1 antikoerper, RHO antikoerper, rhodopsin antikoerper, rhodopsin L homeolog antikoerper, RHO antikoerper, Rho antikoerper, rho antikoerper, rho.L antikoerper
    Substanzklasse
    Chemical
    Hintergrund
    Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
    Gen-ID
    509933
    NCBI Accession
    NP_001014890
    UniProt
    P02699
    Pathways
    WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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