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Phosphotyrosine Antikörper

WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 unconjugated
Produktnummer ABIN863079
  • Target Alle Phosphotyrosine Produkte
    Phosphotyrosine
    Reaktivität
    Bitte anfragen
    Wirt
    • 96
    • 28
    • 1
    Maus
    Klonalität
    • 108
    • 17
    Monoklonal
    Konjugat
    • 44
    • 16
    • 12
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Phosphotyrosine Antikörper ist unkonjugiert
    Applikation
    • 88
    • 64
    • 41
    • 40
    • 40
    • 27
    • 25
    • 17
    • 13
    • 9
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Spezifität
    Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
    Aufreinigung
    Protein G Purified
    Immunogen
    Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
    Klon
    G104
    Isotyp
    IgG1
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN863079 was sufficient for detection of phosphorylated tyrosine residues in 10 μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Target
    Phosphotyrosine
    Abstract
    Phosphotyrosine Produkte
    Substanzklasse
    Amino Acid
    Hintergrund
    Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
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