Caspase 8 Antikörper (Caspase 8, Apoptosis-Related Cysteine Peptidase) (AA 217-221)

Details for Product anti-CASP8 Antibody No. ABIN783312, Anbieter: Anmelden zum Anzeigen
Antigen
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8, apoptosis-related cysteine peptidase
  • death related ced-3/Nedd2-like protein
  • caspase-8
  • caspase 8
  • casp8
  • Dredd
  • CASP8
  • AaeL_AAEL014148
  • LOC100222284
  • Casp8
  • LOC100348477
Epitop
AA 217-221
43
19
18
16
15
12
10
10
9
8
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
360
121
96
25
21
20
3
2
1
Wirt
Kaninchen
278
115
10
2
Klonalität
Polyklonal
Konjugat
Dieser Caspase 8 Antikörper ist unkonjugiert
18
13
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
360
109
92
79
51
36
36
28
14
13
9
8
6
5
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Peptide sequence around aa.217~221 derived from Caspase 8
Sequenz S-E-S-Q-T
Spezifität This antibody detects endogenous level of total Caspase 8 protein.
Kreuzreaktivität (Details) Species reactivity (tested):Human
Reinigung Affinity-chromatography using epitope-specific peptide
Andere Bezeichnung Caspase-8 (CASP8 Antibody Abstract)
Hintergrund Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog
Molekulargewicht 57/45 kDa
Gen-ID 841
NCBI Accession NP_001073593
UniProt Q14790
Forschungsgebiet Apoptosis/Necrosis
Pathways Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store (in aliquots) at -20 °C.
Bilder des Herstellers
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 217-221) antibody (ABIN783312) Western blot analysis of extracts from 293, Hela, HepG2 and HT-29 cells using Caspase 8.
Allgemeine Veröffentlichungen Sánchez, Xu, Juo, Kakizaka, Blenis, Yuan: "Caspase-8 is required for cell death induced by expanded polyglutamine repeats." in: Neuron, Vol. 22, Issue 3, pp. 623-33, 1999 (PubMed).

Varfolomeev, Schuchmann, Luria, Chiannilkulchai, Beckmann, Mett, Rebrikov, Brodianski, Kemper, Kollet, Lapidot, Soffer, Sobe, Avraham, Goncharov, Holtmann, Lonai, Wallach: "Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas/Apo1, and DR3 and is lethal prenatally." in: Immunity, Vol. 9, Issue 2, pp. 267-76, 1998 (PubMed).

Haben Sie etwas anderes gesucht?