SMAD3 Antikörper (pSer423, pSer425)

Produktnummer ABIN746138

Produktdetails anti-SMAD3 Antikörper

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Bindungsspezifität: pSer423, pSer425

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: These phosphorylation sites are homologous across the species listed.

Kreuzreaktivität: Human, Maus, Schwein, Ratte

Homologie: Dog,Cow,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-SMAD3 Antikörper (ABIN746138)

Shang, Liu, Liu, Li, Dou, Zhang, Sun, Zhang, Zhu, Mu, Ding, Wen: "Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-β1." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4537-4544, (2018) (PubMed).

Bai, Chu, Wang, Xiang, Yang: "BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-β signaling." in: Theriogenology, Vol. 100, pp. 24-31, (2017) (PubMed).

Zhang, Yang, Li, Xu, Yan, Gao, Jia, Duan: "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 33, pp. 21-27, (2017) (PubMed).

Shang, Liu, Liu, Zhang, Mu, Zhu, Liang, Zhai, Ding, Li, Wen: "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." in: Scientific reports, Vol. 6, pp. 39809, (2016) (PubMed).

Hu, Duan, Li, Wang, Wang, Chu, Sun, Liu, Wang, Lu, Wen: "Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153409, (2016) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Ghiabi, Jiang, Pasquier, Maleki, Abu-Kaoud, Halabi, Guerrouahen, Rafii, Rafii: "Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche." in: Journal of translational medicine, Vol. 13, Issue 1, pp. 27, (2015) (PubMed).

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: Smad3 + (SMAD3 Produkte)

Synonyme: HSPC193 antikoerper, HsT17436 antikoerper, JV15-2 antikoerper, LDS1C antikoerper, LDS3 antikoerper, MADH3 antikoerper, madh3 antikoerper, madh3a antikoerper, smad3 antikoerper, wu:fa99e03 antikoerper, XSmad3 antikoerper, XenMLP antikoerper, Xmad3 antikoerper, madh3-A antikoerper, Madh3 antikoerper, AU022421 antikoerper, Smad 3 antikoerper, mad3 antikoerper, madh3b antikoerper, zgc:92234 antikoerper, SMAD family member 3 antikoerper, SMAD family member 3a antikoerper, SMAD family member 3 L homeolog antikoerper, SMAD family member 3b antikoerper, SMAD3 antikoerper, smad3a antikoerper, smad3.L antikoerper, Smad3 antikoerper, smad3b antikoerper

Hintergrund:

Synonyms: LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, SMAD family member 3, SMAD 3, Smad3, hSMAD3

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Gen-ID: 4088

UniProt: P84022

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie