Agrin (AGRN) Antikörper

Antigen: Agrin (AGRN)

Synonyme: Agrin, AGR, FLJ45064, AGRN, AGRIN, agrn, wu:fb57e07

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN738786

Produktbeschreibung

Weitere Bezeichnung: Agrin

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Agrin C-terminus

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Synonym: AGRIN, Agrin proteoglycan, AGRN, FLJ45064, OTTHUMP00000044043.

Molekulargewicht: 208kDa

Kommentare:

Neuron marker Agrin is a neuronal aggregating factor that induces the aggregation of acetylcholine receptors and other postsynaptic proteins on muscle fibers and is crucial for the formation of the neuromuscular junction. Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin

MuSK and Rapsyn are all essential components for AChR aggregation

through an unknown mechanism. The C-terminal region of agrin is released into the medium

interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites

increasing the predicted MW from 200kDa to ~600kDa.

Anwendungen

Applikationshinweise: ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Neurologie

Beschränkungen: Nur für Forschungszwecke einsetzbar