Atp1b2/Na+K+Atpase (AA 201-290) Antikörper

Produktnummer ABIN738426

Produktdetails

Target: Atp1b2/Na+K+Atpase

Bindungsspezifität: AA 201-290

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow,Pig,Horse,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATP1b2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #100012 (Immunofluorescence)

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100012

Datum: 11.05.2016

Antigen: Atp1b2/Na+K+Atpase (ATP1B2)

Validierte Anwendung: Immunofluorescence

Positivkontrolle: Wild-type mouse adult brain cryo-sections

Negativkontrolle: Rabbit IgG

Bewertung: The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.

Validierungsbilder

IF validation of ATP1B2 antibody ABIN738426 on mouse brain sections. A. ABIN738426 diluted 1:400 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:200. D. Dilution factor 1:400.

Protokoll

Primärantikörper: ABIN738426

Sekundärantikörper: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• • Dry slides at 50-55°C for 15-30min (or RT for 30min).
  • Wash with PBS 5min X 3 (shake gently).
  • Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
  • Incubate with ABIN738426 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
  • Wash with PBS 5min X 3.
  • Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
  • Wash with PBS 5min X 3.
  • Add three drops of water-based Immu-mount.
  • Cover with 24 X 60 cover glass.
• Imaging:
• • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Anmerkungen: ABIN738426 worked within 1:50–1:400, worked well at 1:400.

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Atp1b2/Na+K+Atpase

Hintergrund:

Synonyms: AMOG, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule in glia, Sodium/potassium-dependent ATPase subunit beta-2, ATP1B2

Background: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.

Gen-ID: 482

UniProt: P14415