MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (AA 440-491)

Details for Product anti-MDM2 Antibody No. ABIN736821, Anbieter: Anmelden zum Anzeigen
Antigen
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
Epitop
AA 440-491
46
42
36
29
26
24
15
15
14
12
9
8
7
7
6
5
5
5
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
378
135
94
14
8
6
4
2
2
2
2
2
2
1
1
1
Wirt
Kaninchen
305
77
14
2
Klonalität
Polyklonal
Konjugat
Dieser MDM2 Antikörper ist unkonjugiert
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
262
136
108
84
71
39
38
25
13
12
12
12
4
4
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Chargennummer 110602
Validierte Anwendung Immunofluorescence
Positivkontrolle Human breast carcinoma
Negativkontrolle Pig muscle tissue (non-reactive species)
Bewertung Signal was detected in positive control sample and not in negative control sample. Note low levels of background in negative control and isotype control samples.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: FFPE human breast carcinoma block from Molecular Pathology Core.
  • Negative Control: FFPE Pig skeletal muscle block from Molecular Pathology Core.
  • Secondary antibody only control: Human breast carcinoma treated with Goat anti-Rabbit AF488 secondary antibody only.
  • Isotype control: FFPE human Breast Carcinoma block from Molecular Pathology Core.
Protokoll
  • Human breast carcinoma and pig skeletal muscle (formalin fixed paraffin embedded) blocks were from Molecular Pathology Core.
  • The 4 uM thick sections were treated by Citra in steamer for 20 min and cool down on the bench for another 20 min.
  • Sections were blocked in 1 X TBS / 5% normal goat serum for 2 h at RT.
  • Sections were incubated with primary antibody diluted 1:100 in 1xTBS overnight at 4°C.
  • Sections were rinsed three times in TBS for 5 min each at RT.
  • Sections were incubated with secondary antibody diluted 1:1000 in 1xTBS for 60 min at RT in dark.
  • Sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI)
  • Stained sections were imaged with a Zeiss Axioskop2 microscope.
Anmerkungen - MDM2 on pig tissues were negative and MDM2 on human breast CA tissues showed nuclei staining, although with some faint cytoplasmic staining.
Validierungsbilder
Immunofluorescence validation image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 440-491) antibody (ABIN736821) Figure 1: Human breast cancer tissue stained with anti-MDM2 (green) and counterstaine...
Immunofluorescence validation image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 440-491) antibody (ABIN736821) Figure 2: Pig muscle tissue stained with anti-MDM2 (green) and counterstained with DA...
Immunofluorescence validation image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 440-491) antibody (ABIN736821) Figure 3: Human breast cancer tissue stained with isotype control antibody (green) an...
Immunofluorescence validation image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 440-491) antibody (ABIN736821) Figure 1: Human breast cancer tissue stained with secondary antibody only (green) and...
Immunogen KLH conjugated synthetic peptide derived from human MDM2
Isotyp IgG
Reinigung Purified by Protein A.
Andere Bezeichnung Mdm2 (MDM2 Antibody Abstract)
Hintergrund

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation.

Subcellular location: Cytoplasm, Nucleus

Synonyms: HDMX, hdm2, ACTFS, E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2

Gen-ID 4193
UniProt Q00987
Forschungsgebiet Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 440-491) antibody (ABIN736821) Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-MDM2 Polyclo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 440-491) antibody (ABIN736821) Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-MDM2 Polyclo...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 440-491) antibody (ABIN736821) Images provided the Independent Validation Program (badge number 029683)Formalin-fixe...
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