M-CSF Antikörper

Antigen: M-CSF

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Ratte (Rattus), Human

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN736806

Produktbeschreibung

Weitere Bezeichnung: MCSF

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from mouse MCSF C-terminus

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Synonym: Macrophage Colony Stimulating Factors, Colony stimulating factor 1 (macrophage), Colony stimulating factor 1, Colony stimulating factor macrophage specific, CSF 1, CSF1, Csfm, Lanimostim, M CSF, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930.

Molekulargewicht: 60kDa

Kommentare:

Four distinct colony-stimulating factors (CSFs) that promote survival

proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF)

granulocyte CSF (GCSF)

macrophage CSF (MCSF)

and Interleukin-3 (IL-3

Multi CSF). Both GMCSF and IL-3 are multipotential growth factors

stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast

GCSF and MCSF are lineage restricted hematopoietic growth factors

stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors.Macrophage CSF

also known as CSF1

is produced by monocytes

fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies

enhances antibody-dependent

cell-mediated cytotoxicity by monocytes and macrophages

and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight

existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III

a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).

Anwendungen

Applikationshinweise: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Kardiovaskuläres System, Artherosklerose, Angeborene Immunität, Zytokine

Beschränkungen: Nur für Forschungszwecke einsetzbar