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Elastin Antikörper (AA 681-786)

ELN Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p), FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN734003
  • Target Alle Elastin (ELN) Antikörper anzeigen
    Elastin (ELN)
    Bindungsspezifität
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    AA 681-786
    Reaktivität
    • 57
    • 23
    • 9
    • 8
    • 7
    • 7
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 50
    • 16
    • 1
    Kaninchen
    Klonalität
    • 48
    • 19
    Polyklonal
    Konjugat
    • 34
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    Dieser Elastin Antikörper ist unkonjugiert
    Applikation
    • 28
    • 21
    • 15
    • 14
    • 12
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)
    Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Elastin
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #028751 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Beth Israel Deaconess Medical Center Confocal Imaging Core
    No.
    #028751
    Datum
    25.09.2013
    Antigen
    Chargennummer
    120306
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle
    Mouse heart tissue
    Negativkontrolle
    Mouse adipose tissue
    Bewertung
    Signal was detected in positive control tissue and not detected in negative control tissue.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antibody: Elastin (ELN) (C-Term) antibody
    • Catalog number: ABIN734003
    • Lot number: 120306
    Sekundärantikörper
    • Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)
    Full Protocol
    • Immunohistochemistry was performed manually.
    • Sections were deparaffinized and rehydrated.
    • Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
    • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
    • Sections were washed x 3 in Tris buffered saline (TBS).
    • Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
    • Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
    • Sections were washed x 3 in Tris buffered saline.
    • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
    • Sections were washed x 3 in Tris buffered saline.
    • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
    • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
    • Sections were washed x 3 in Distilled Water.
    • Sections were counterstained with hematoxylin for 1 min.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
    Anmerkungen
    None
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Hasegawa, Sato, Shirai, Watanabe, Yamamoto, Watanabe, Nohtomi, Hirano, Watanabe: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." in: PLoS ONE, Vol. 9, Issue 12, pp. e110866, (2014) (PubMed).

    Konii, Sato, Kikuchi, Okiyama, Watanabe, Hasegawa, Yamamoto, Itoh, Hirano, Watanabe: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." in: Hypertension, Vol. 62, Issue 5, pp. 942-50, (2013) (PubMed).

    Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).

    Ikeda, Yada, Takase: "[Syntheses of 1,3,4-thiadiazole derivatives]." in: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, (1970) (PubMed).

  • Target
    Elastin (ELN)
    Andere Bezeichnung
    Elastin (ELN Produkte)
    Synonyme
    SVAS antikoerper, WBS antikoerper, WS antikoerper, AI385707 antikoerper, AI480567 antikoerper, E030024M20Rik antikoerper, RATTREL11 antikoerper, TREL11 antikoerper, Trela antikoerper, Trela26 antikoerper, ELN antikoerper, elastin antikoerper, ELN antikoerper, Eln antikoerper, LOC574132 antikoerper, Tcur_2481 antikoerper, Kfla_2289 antikoerper, LOC100620140 antikoerper
    Hintergrund

    Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN

    Background: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

    Gen-ID
    2006
    UniProt
    P15502
    Pathways
    Regulation of Actin Filament Polymerization
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