Elastin (ELN) (AA 750-786) Antikörper

Details zu Produkt Nr. ABIN734003, Anbieter: Anmelden zum Anzeigen
Antigen
  • ELN
  • SVAS
  • WBS
  • WS
  • RATTREL11
  • TREL11
  • Trela
  • Trela26
  • AI385707
  • AI480567
  • E030024M20Rik
  • elastin
  • elastin (supravalvular aortic stenosis, Williams-Beuren syndrome)
  • elastin-like
  • ELN
  • Tcur_2481
  • Kfla_2289
  • Eln
  • LOC100620140
Epitop
AA 750-786
8
6
5
2
2
2
2
1
Reaktivität
Human, Maus, Ratte (Rattus)
67
22
18
15
13
13
5
4
3
3
3
3
1
Wirt
Kaninchen
58
29
2
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
4
3
2
2
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
57
42
29
26
14
11
10
9
8
3
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Chargennummer 120306
Validierte Anwendung Immunohistochemistry
Positivkontrolle Mouse heart tissue
Negativkontrolle Mouse adipose tissue
Bewertung Signal was detected in positive control tissue and not detected in negative control tissue.
Primärantikörper
  • Antibody: Elastin (ELN) (C-Term) antibody
  • Catalog number: ABIN734003
  • Lot number: 120306
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Wild type mouse heart tissue (specimen known to contain the target protein).
  • Negative control: Wild type adipose tissue (specimen known to not contain the target protein).
  • Primary antibody isotype control: Wild type mouse heart treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse heart treated with secondary antibody only (no
  • primary antibody).
Protokoll
  • Immunohistochemistry was performed manually.
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
  • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
  • Sections were washed x 3 in Tris buffered saline (TBS).
  • Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
  • Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
  • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin for 1 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
Anmerkungen None
Validierungsbilder
Immunohistochemistry validation image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue...
Immunogen KLH conjugated synthetic peptide derived from human Elastin
Isotyp IgG
Reinigung Purified by Protein A.
Andere Bezeichnung Elastin (ELN Antibody Abstract)
Hintergrund

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Subcellular location: Extracellular, Secreted

Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN

Gen-ID 2006
UniProt P15502
Forschungsgebiet Cardiovascular, Extracellular Matrix
Pathways Regulation of Actin Filament Polymerization
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclona...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclona...
SDS-PAGE (SDS) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclo...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTI...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Images provided the Independent Validation Program (badge number 28751): Formalin-fix...
Flow Cytometry (FACS) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody
Western Blotting (WB) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated at 1:300 ov...
Produkt verwendet in: Hasegawa, Sato, Shirai, Watanabe, Yamamoto, Watanabe, Nohtomi, Hirano, Watanabe: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." in: PLoS ONE, Vol. 9, Issue 12, pp. e110866, 2014 (PubMed).

Konii, Sato, Kikuchi, Okiyama, Watanabe, Hasegawa, Yamamoto, Itoh, Hirano, Watanabe: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." in: Hypertension, Vol. 62, Issue 5, pp. 942-50, 2013 (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, 2012 (PubMed).

Ikeda, Yada, Takase: "[Syntheses of 1,3,4-thiadiazole derivatives]." in: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, 1970 (PubMed).

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