O6-Methylguanine-DNA-Methyltransferase (MGMT) Antikörper (Biotin)

Antigen: O6-Methylguanine-DNA-Methyltransferase (MGMT)

Synonyme: MGMT

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Konjugat: Biotin

Applikation: Western Blot (WB), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN730770

Produktbeschreibung

Weitere Bezeichnung: MGMT

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human MGMT C-terminus

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG

Beschreibung: Synonym: 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, EC 2.1.1.63, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, MGC107020, MGMT: O-6 methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase, O-6 methylguanine DNA alkyltransferase, O-6-methylguanine-DNA methyltransferase, O6 methylguanine DNA methyltransferase.

Molekulargewicht: 23kDa

Kommentare:

MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine

and other methylated moities of DNA

to a cysteine residue in itself

thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation

and also provides resistance to chemotherapy with alkylating agents among cancer patients.

Anwendungen

Applikationshinweise: WB (1:100-500), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS, Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar