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AQP2 Antikörper (AA 171-271)

AQP2 Reaktivität: Human, Ratte, Maus WB, ELISA, IF (p), IF (cc), ICC, IHC (p), FACS, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN707576
  • Target Alle AQP2 Antikörper anzeigen
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    Bindungsspezifität
    • 22
    • 20
    • 17
    • 17
    • 15
    • 15
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 171-271
    Reaktivität
    • 112
    • 94
    • 84
    • 16
    • 16
    • 11
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human, Ratte, Maus
    Wirt
    • 133
    • 2
    Kaninchen
    Klonalität
    • 133
    • 3
    Polyklonal
    Konjugat
    • 50
    • 10
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser AQP2 Antikörper ist unkonjugiert
    Applikation
    • 125
    • 40
    • 40
    • 39
    • 29
    • 21
    • 20
    • 18
    • 18
    • 14
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Kreuzreaktivität
    Human, Maus, Ratte
    Homologie
    Dog
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human AQP2.
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029457 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029457
    Datum
    16.01.2014
    Antigen
    Chargennummer
    999994W
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle
    - Human kidney tubules - Human breast ductal epithelium
    Negativkontrolle
    - Human kidney glomeruli and stromal tissue - Human breast mesenchymal tissue
    Bewertung
    Strong signal was observed in positive control tissues and not in negative control tissues.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antibody: Aquaporin 2
    • Catalog number: ABIN707576
    • Batch number: 999994W
    Sekundärantikörper
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D05923BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 min
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
    • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
    • 4. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 5. Slides rinsed with reaction buffer x3
    • 6. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 min
    • 7. Slides rinsed with *reaction buffer*
    • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 µL applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
    • 9. Slides rinsed with *reaction buffer* x3
    • 10. *iVIEW Biotinylated IgG* applied and incubated for 8 min
    • 11. Slides rinsed with *reaction buffer*
    • 12. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
    • 13. Slides rinsed with *reaction buffer*
    • 14. *iVIEW DAB/H2O2* applied and incubated for 8 min
    • 15. Slides rinsed with *reaction buffer*
    • 16. *iVIEW Copper* applied and incubated for 4 min
    • 17. Slides rinsed with *reaction buffer*
    • 18. Slides washed in Dawn Detergent/tap water
    • 19. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
    • 20. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
    • 21. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Anmerkungen
    • Deviations from protocol/procedure supplied by manufacturer.
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Lazzeri, Angelotti, Peired, Conte, Marschner, Maggi, Mazzinghi, Lombardi, Melica, Nardi, Ronconi, Sisti, Antonelli, Becherucci, De Chiara, Guevara, Burger, Schaefer, Annunziato, Anders, Lasagni et al.: "Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury. ..." in: Nature communications, Vol. 9, Issue 1, pp. 1344, (2018) (PubMed).

  • Target
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    Andere Bezeichnung
    AQP2 (AQP2 Produkte)
    Synonyme
    AQP-CD antikoerper, WCH-CD antikoerper, cph antikoerper, jpk antikoerper, AQP-2 antikoerper, aquaporin-2 antikoerper, aquaporin 2 antikoerper, aquaporin 2 (collecting duct) antikoerper, Aqp2 antikoerper, AQP2 antikoerper
    Hintergrund

    Synonyms: AQP-CD, WCH-CD, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct, AQP2

    Background: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

    Gen-ID
    359
    UniProt
    P41181
    Pathways
    Response to Water Deprivation
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