BAP1 Antikörper (pSer592)

Produktnummer ABIN683413

Produktdetails anti-BAP1 Antikörper

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Bindungsspezifität: pSer592

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAP1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This phosphorylation site is homologous to the Ser590 site in Rat.

Kreuzreaktivität: Ratte

Homologie: Human,Dog,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BAP1 around the phosphorylation site of Ser592

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu BAP1

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Andere Bezeichnung: BAP1 (BAP1 Produkte)

Synonyme: HUCEP-13 antikoerper, UCHL2 antikoerper, BAIAP3 antikoerper, 2300006C11Rik antikoerper, AA989761 antikoerper, AW553466 antikoerper, mKIAA0272 antikoerper, uch-x4 antikoerper, hucep-13 antikoerper, hucep-6 antikoerper, uchl2 antikoerper, si:dkey-42i9.9 antikoerper, BRCA1 associated protein 1 antikoerper, Brca1 associated protein 1 antikoerper, BRCA1 associated protein 1 L homeolog antikoerper, BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) antikoerper, BAP1 antikoerper, Bap1 antikoerper, bap1.L antikoerper, bap1 antikoerper

Hintergrund:

Synonyms: UCHL2, hucep-6, HUCEP-13, Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1, Cerebral protein 6, BAP1, KIAA0272

Background: Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Acts as a tumor suppressor.

Gen-ID: 8314

UniProt: Q92560