MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492)

Details for Product anti-FRAP1 Antibody No. ABIN676403, Anbieter: Anmelden zum Anzeigen
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
AA 2436-2492
66
36
28
23
21
15
14
13
12
10
8
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
375
199
197
18
9
8
7
7
7
7
4
2
2
2
1
1
Wirt
Kaninchen
323
48
3
1
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
8
8
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
252
114
98
73
57
51
37
21
8
7
7
6
3
2
2
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
'Independent Validation' Siegel
Chargennummer 131209
Validierte Anwendung Western Blotting
Positivkontrolle HeLa cells
Negativkontrolle c6/36 mosquito cells (non-reactive species)
Bewertung A strong band was observed in the positive control sample at the correct molecular weight. Multiple faint bands were also observed in the positive sample, but these bands did not represent a significant background signal. A band at the correct molecular weight was also observed in the negative control; however, it may be impossible to find a true negative control for this antigen.
Primärantikörper
  • Antigen: Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (1:200 dilution)
  • Catalog number: ABIN676403
  • Supplier: Anmelden zum Anzeigen
  • Supplier catalog number: Anmelden zum Anzeigen
  • Lot number: 131209
Sekundärantikörper
Kontrollen
  • Positive control: HeLa cell extract
  • Negative control: c6/36 Mosquito cell extract
Protokoll
  • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
  • The membrane was rinsed with TBST once.
  • The membrane was immersed with the protein side up in the primary antibody solution (FRAP1; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
  • The membrane was rinsed in TBST thrice for 5 min each.
  • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • The membrane was rinsed thrice TBST thrice for 5 min each.
  • The membrane was rinsed in TBS twice for 30 s each.
  • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • The membrane was rinsed three times TBST.
  • Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • The membrane was washed in TBST 2 times for 10 min each.
  • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Anmerkungen - No experimental challenges noted.
Validierungsbilder
Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2436-2492) antibody (ABIN676403) Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) p...
Immunogen KLH conjugated synthetic peptide derived from human mTOR
Isotyp IgG
Reinigung Purified by Protein A.
Andere Bezeichnung Mtor/Frap (FRAP1 Antibody Abstract)
Hintergrund

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Synonyms: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Gen-ID 2475
UniProt P42345
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events
Applikationshinweise IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2436-2492) antibody (ABIN676403) Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Pol...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2436-2492) antibody (ABIN676403) Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2436-2492) antibody (ABIN676403) Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Pol...
Produkt verwendet in: Zou, Geng, Chen, Ren, Liu, Wan, Guo, Wang: "Ranolazine improves oxidative stress and mitochondrial function in the atrium of acetylcholine-CaCl2 induced atrial fibrillation rats." in: Life sciences, Vol. 156, pp. 7-14, 2016 (PubMed).

Madka, Mohammed, Li, Zhang, Biddick, Patlolla, Lightfoot, Towner, Wu, Steele, Kopelovich, Rao: "Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo." in: Cancer prevention research (Philadelphia, Pa.), Vol. 9, Issue 1, pp. 53-62, 2016 (PubMed).

Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." in: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, 2015 (PubMed).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014 (PubMed).

Haben Sie etwas anderes gesucht?