WNT2B Antikörper (Wingless-Type MMTV Integration Site Family, Member 2B) (AA 341-391)

Details for Product anti-WNT2B Antibody No. ABIN675728, Anbieter: Anmelden zum Anzeigen
Antigen
  • wnt2b
  • WNT2B
  • Wnt13
  • WNT13
  • XWNT2
  • XWnt-2
  • Xwnt-2b
  • wnt-2b
  • xwnt2b
  • wingless-type MMTV integration site family, member 2Ba
  • wingless-type MMTV integration site family, member 2B
  • wingless related MMTV integration site 2b
  • wnt2ba
  • WNT2B
  • LOC100227493
  • LOC100349847
  • Wnt2b
  • wnt2b-a
Epitop
AA 341-391
15
14
7
6
2
2
2
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
64
34
32
13
12
11
11
10
9
8
7
5
2
1
1
1
Wirt
Kaninchen
63
2
Klonalität
Polyklonal
Konjugat
Dieser WNT2B Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
32
24
14
12
5
3
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Chargennummer 120522
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human kidney
Negativkontrolle Human liver
Bewertung Faint but distinguishable signal was detected in positive control sample and not in negative control sample.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Human kidney tissue stained with antibody
  • Negative control: Human liver tissue stained with antibody
  • Isotype control: Human kidney tissue stained with isotype control
  • Secondary only control: Human kidney tissue stained with secondary antibody only
Protokoll
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validierungsbilder
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 1: Human kidney tissue stained with anti-WNT2 (brown) and counterstained with ...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 2: Human liver tissue stained with anti-WNT2 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 3: Human kidney tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 1: Human kidney tissue stained with secondary antibody only (brown) and counte...
Immunogen KLH conjugated synthetic peptide derived from human WNT2B
Isotyp IgG
Reinigung Purified by Protein A.
Andere Bezeichnung Wnt2b (WNT2B Antibody Abstract)
Hintergrund

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

Subcellular location: Secreted

Synonyms: WNT13, Protein Wnt-2b, Protein Wnt-13, WNT2B

Gen-ID 7482
UniProt Q93097
Forschungsgebiet Cancer, Wnt
Pathways WNT Signalweg
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Lane 1: rat brain lysates Lane 2: mouse intestine lysates probed with Anti WNT2B AVPR...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Independently Validated Antibody, image provided by Science Direct, badge number 0294...
Produkt verwendet in: Li, Liu, Su, Ren, Zhu, Tian, Qiu: "MicroRNA-324-3p regulates nasopharyngeal carcinoma radioresistance by directly targeting WNT2B." in: European journal of cancer (Oxford, England : 1990), Vol. 49, Issue 11, pp. 2596-607, 2014 (PubMed).

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